Aime S, Dastrú W, Fasano M, Arnelli A, Castagnola M, Giardina B, Ascenzi P
Dipartimento di Chimica Inorganica, Università di Torino, Italy.
Clin Chem. 1992 Dec;38(12):2401-4.
We report a new method for the quantitative determination of human methemoglobin (metHb) based on the measurement of the solvent-water proton-nuclear magnetic resonance (NMR) relaxation rate R1 [normalized to 1 mmol/L hemoglobin (Hb) concentration]. MetHb (%) is estimated from the linear dependence of R1 on the metHb concentration, taking into account the simple relationship [MetHb] = [(R1 - R1HbO2)/(R1metHb - R1HbO2)].100, where R1HbO2 and R1metHb are values for the solvent-water relaxation rate of standard 1.0 mmol/L solutions of the oxygenated derivative of human hemoglobin (HbO2) and of metHb, respectively. The minimum metHb that may be determined from the analysis of the experimental data is 0.5 +/- 0.4%.
我们报告了一种基于溶剂水质子核磁共振（NMR）弛豫率R1（归一化为1 mmol/L血红蛋白（Hb）浓度）定量测定人体高铁血红蛋白（metHb）的新方法。根据R1与metHb浓度的线性关系估算metHb（%），同时考虑简单关系[MetHb] = [(R1 - R1HbO2)/(R1metHb - R1HbO2)]×100，其中R1HbO2和R1metHb分别是人体血红蛋白氧化衍生物（HbO2）和metHb的1.0 mmol/L标准溶液的溶剂水弛豫率值。根据实验数据分析可测定的最低metHb为0.5±0.4%。
