基于细菌人工染色体的中枢神经系统基因表达图谱。

A gene expression atlas of the central nervous system based on bacterial artificial chromosomes.

作者信息

Gong Shiaoching, Zheng Chen, Doughty Martin L, Losos Kasia, Didkovsky Nicholas, Schambra Uta B, Nowak Norma J, Joyner Alexandra, Leblanc Gabrielle, Hatten Mary E, Heintz Nathaniel

机构信息

GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA.

出版信息

Nature. 2003 Oct 30;425(6961):917-25. doi: 10.1038/nature02033.

DOI:10.1038/nature02033

PMID:14586460

Abstract

The mammalian central nervous system (CNS) contains a remarkable array of neural cells, each with a complex pattern of connections that together generate perceptions and higher brain functions. Here we describe a large-scale screen to create an atlas of CNS gene expression at the cellular level, and to provide a library of verified bacterial artificial chromosome (BAC) vectors and transgenic mouse lines that offer experimental access to CNS regions, cell classes and pathways. We illustrate the use of this atlas to derive novel insights into gene function in neural cells, and into principal steps of CNS development. The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community.

摘要

哺乳动物的中枢神经系统（CNS）包含大量不同的神经细胞，每个神经细胞都有复杂的连接模式，这些连接模式共同产生感知和更高层次的脑功能。在这里，我们描述了一项大规模筛选，旨在创建细胞水平上的中枢神经系统基因表达图谱，并提供一个经过验证的细菌人工染色体（BAC）载体和转基因小鼠品系文库，这些载体和品系可用于实验研究中枢神经系统区域、细胞类型和信号通路。我们举例说明了如何利用该图谱获得关于神经细胞基因功能以及中枢神经系统发育主要步骤的新见解。在此筛选中生成的图谱、BAC载体文库和BAC转基因小鼠提供了丰富的资源，使得神经科学界以前无法进行的广泛研究成为可能。