Chen Z Y, Jiang X Y, Fei L
Department of Pediatrics, Second Affiliated Hospital, Hubei Medical College, Wuhan.
Chin Med J (Engl). 1992 Aug;105(8):671-5.
The effect of heparin on the anionic sites of glomerular basement membrane (GBM) in adriamycin-induced nephrotic rats was studied by means of calculating the polyethyleneimine (PEI) stained particles under electron microscope. The results showed that treatment with heparin under the anticoagulant effect markedly reduced the loss of anionic sites within lamina rara externa (LRE) of GBM and also the urinary protein excretion in nephrotic rats. The results demonstrated that one of the mechanisms of heparin in preventing the structural damage of glomeruli in kidney diseases was the reduction of the loss of anionic sites within LRE, indicating that changes in biomembrane charge played an important role in the pathogenesis of nephrotic syndrome. The results provided a scientific basis for the use of heparin to treat glomerular diseases.
通过电子显微镜下计算聚乙烯亚胺（PEI）染色颗粒，研究了肝素对阿霉素诱导的肾病大鼠肾小球基底膜（GBM）阴离子位点的影响。结果显示，在抗凝作用下用肝素治疗可显著减少GBM外疏松层（LRE）中阴离子位点的丢失，以及肾病大鼠的尿蛋白排泄。结果表明，肝素预防肾脏疾病中肾小球结构损伤的机制之一是减少LRE中阴离子位点的丢失，这表明生物膜电荷变化在肾病综合征的发病机制中起重要作用。这些结果为使用肝素治疗肾小球疾病提供了科学依据。
