Gray L E, Ostby J S, Kavlock R J, Marshall R
Developmental Reproductive Biology Section, Reproductive Toxicology Branch/HERL/USEPA, Research Triangle Park, North Carolina.
Fundam Appl Toxicol. 1992 Oct;19(3):411-22. doi: 10.1016/0272-0590(92)90180-p.
The present study describes the relationship between gonadal agenesis and fertility in male and female mice exposed in utero to the diazo dye Congo red (CR). Maternal CR treatment inhibited testicular and ovarian function in the offspring after oral administration of 1 or 0.5 g/kg/day on Gestational Days 8-12. The testes of male offspring from CR-exposed dams were small in size and contained hypospermatogenic seminiferous tubules. However, despite the fact that testis weight was reduced by more than 70% in some males, they displayed normal levels of fertility when mated to untreated females for over 10 months. In contrast, female offspring from CR-exposed dams produced only about half as many litters and pups as the control pairs did under long-term mating conditions. Histological examination of the ovaries revealed that subfertility was correlated with ovarian atrophy. Females lacking maturing follicles were considerably less productive (1.3 litters and 11.5 pups) than treated females with histologically normal ovaries (7.1 litters and 78.1 pups). In summary, prenatal exposure to the dye CR affects the gonads of both male and female offspring, but only the female offspring display reduced fertility.
本研究描述了子宫内暴露于重氮染料刚果红（CR）的雄性和雌性小鼠性腺发育不全与生育能力之间的关系。在妊娠第8 - 12天口服给予1或0.5 g/kg/天的CR后，母体CR处理抑制了后代的睾丸和卵巢功能。来自暴露于CR的母鼠的雄性后代的睾丸体积小，生精小管生精功能低下。然而，尽管在一些雄性中睾丸重量减少了70%以上，但当与未处理的雌性交配超过10个月时，它们的生育能力水平正常。相比之下，在长期交配条件下，来自暴露于CR的母鼠的雌性后代产仔数和幼崽数仅约为对照组的一半。卵巢组织学检查显示，生育力低下与卵巢萎缩相关。缺乏成熟卵泡的雌性（1.3窝和11.5只幼崽）比卵巢组织学正常的经处理雌性（7.1窝和78.1只幼崽）的生育能力明显更低。总之，产前暴露于染料CR会影响雄性和雌性后代的性腺，但只有雌性后代的生育能力降低。
