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酵母RNA聚合酶II转录系统中染色质模板上的起始过程。

Initiation on chromatin templates in a yeast RNA polymerase II transcription system.

作者信息

Lorch Y, LaPointe J W, Kornberg R D

机构信息

Department of Cell Biology, Stanford University School of Medicine, California 94305.

出版信息

Genes Dev. 1992 Dec;6(12A):2282-7. doi: 10.1101/gad.6.12a.2282.

DOI:10.1101/gad.6.12a.2282
PMID:1459452
Abstract

Templates were prepared with either the TATA box or transcription start sites of the yeast CYC1 promoter in a nucleosome. In both cases, initiation in an unfractionated yeast RNA polymerase II transcription system was abolished by the nucleosome. The inhibition appeared to be relieved by the activator protein Gal4-VP16 binding to a site upstream of the promoter. Inhibition was not relieved, however, in a transcription system reconstituted from purified components, indicating a requirement for additional factors for the effect of Gal4-VP16.

摘要

模板是用酵母CYC1启动子的TATA盒或转录起始位点在核小体中制备的。在这两种情况下，未分级的酵母RNA聚合酶II转录系统中的起始都被核小体所抑制。这种抑制似乎通过激活蛋白Gal4-VP16与启动子上游的位点结合而得到缓解。然而，在由纯化成分重构的转录系统中，抑制并未得到缓解，这表明Gal4-VP16发挥作用还需要其他因子。

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