对接种重组莫洛尼鼠白血病病毒-TB的BALB/c小鼠来源的连续淋巴细胞系的特性鉴定
Characterization of a continuous lymphocyte cell line derived from BALB/c mice inoculated with a recombinant Moloney murine leukemia virus-TB.
作者信息
Prasad G, Wong P K, Yuen P H
机构信息
UTMD Anderson Cancer Centre, Science Park Research Division, Smithville.
出版信息
Indian J Exp Biol. 1992 May;30(5):357-61.
Neonatal BALB/c mice were inoculated (ip) with a recombinant Moloney murine leukemia virus-TB. Majority of the inoculated mice developed lymphoma within 5-7 months post infection. The cells from splenic lymphomas were cultured and 3 continuous cell lines (GP1, GP2 and GP3) developed. GP1 was single cell cloned and characterized. Based on Thy 1.2 (98.4%) phenotypic marker, the cell line was categorized as T cell line. The percent positivity for different cell surface markers on analysis with FACS was 98.4, 4.8, 5.5, 2.2, 1.8, 1.2 and 9.5 for Thy 1.2, mu, L3T4, Lyt2, Ia, IL2R and PNA receptor, respectively. A total of 16.5% GP1 cells was also positive for Moloney murine leukemia virus envelope protein (gp 70). Incomplete retrovirus like particles were demonstrated in the cytoplasm of GP1 cells by electron microscopy. The cell line on inoculation(ip) in neonatal BALB/c mice produced lymphomic lesions in almost all the vital organs of the mice.
将重组莫洛尼鼠白血病病毒-TB经腹腔接种新生BALB/c小鼠。大多数接种小鼠在感染后5-7个月内发生淋巴瘤。培养来自脾脏淋巴瘤的细胞，建立了3个连续细胞系（GP1、GP2和GP3）。对GP1进行单细胞克隆并鉴定其特征。基于Thy 1.2（98.4%）表型标志物，该细胞系被归类为T细胞系。用流式细胞仪分析不同细胞表面标志物的阳性百分比，Thy 1.2、mu、L3T4、Lyt2、Ia、IL2R和PNA受体分别为98.4%、4.8%、5.5%、2.2%、1.8%、1.2%和9.5%。总共16.5%的GP1细胞对莫洛尼鼠白血病病毒包膜蛋白（gp 70）也呈阳性。通过电子显微镜在GP1细胞的细胞质中发现了不完全逆转录病毒样颗粒。将该细胞系经腹腔接种新生BALB/c小鼠后，在小鼠几乎所有重要器官中产生淋巴瘤病变。
Zotero 插件