通过毛细管电泳法测定马兜铃酸

Determination of aristolochic acids by capillary electrophoresis.

作者信息

Priestap Horacio A, Iglesias Silvia L, Desimone Martín F, Diaz Luis E

机构信息

Cátedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

出版信息

J Capill Electrophor Microchip Technol. 2003 May-Aug;8(3-4):39-43.

PMID:14596334

Abstract

A method for the separation and quantification of aristolochic acids by capillary electrophoresis is described. Buffer solutions composed of sodium dihydrogen phosphate, sodium borate, and sodium dodecyl sulfate at pH 6.5-7 were found to be suitable for the separation of aristolochic acids, which can be well resolved in a few minutes. The separation and identification of six aristolochic acids contained in a commercial tincture called charrua, a traditional herbal medicine consisting of a hydroalcoholic extract of Aristolochia argentina, corroborate the usefulness of the method. The eventual toxic properties of the aristolochic acid containing charrua tincture are discussed.

摘要

本文描述了一种通过毛细管电泳分离和定量马兜铃酸的方法。发现由磷酸二氢钠、硼酸钠和十二烷基硫酸钠组成的pH值为6.5 - 7的缓冲溶液适合分离马兜铃酸，马兜铃酸能在几分钟内得到很好的分离。对一种名为查鲁阿的商业酊剂（一种由阿根廷马兜铃水醇提取物制成的传统草药）中所含的六种马兜铃酸进行分离和鉴定，证实了该方法的有效性。文中还讨论了含马兜铃酸的查鲁阿酊剂可能的毒性。