Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
J Pharm Biomed Anal. 2010 Aug 1;52(4):446-51. doi: 10.1016/j.jpba.2010.01.023. Epub 2010 Jan 20.
A novel method has been developed for the purification of aristolochic acids and aristololactams compounds from Aristolochia plants, a kind of typically toxic traditional Chinese medicine. In this method, Oligo (ethylene glycol) separation column which has "clustering function" for compounds in TCMs was used to produce the fractions containing the compounds with similar structures. A four-channel parallel preparative HPLC with C18 separation column was employed to purify the target compounds. The extraction sample of the blending of Radix Aristolochiae, Fructus Aristolochiae and Caulis Aristolochiae Manshuriensis was used to develop the method. Then, four aristolochic acids and three aristololactams were obtained using this method and the chemical identification was confirmed by Q-TOF-MS, (1)H NMR and (13)C NMR. Thus, this method can deal with more than one traditional Chinese medicine simultaneously. Additionally, the results demonstrated that this method was an effective way to purify target compounds selectively from TCMs.
一种从关木通、广防己和马兜铃等具有典型毒性的中药材中提取马兜铃酸和马兜铃内酰胺类化合物的新方法。该方法采用“聚类功能”的 Oligo(乙二醇)分离柱对中药化合物进行分段，得到具有相似结构的化合物的馏分。采用四通道平行制备 HPLC 用 C18 分离柱对目标化合物进行纯化。该方法以关木通、防己和马兜铃的混合提取物为样品进行开发。然后，使用该方法得到了四种马兜铃酸和三种马兜铃内酰胺，并用 Q-TOF-MS、(1)H NMR 和 (13)C NMR 进行了化学鉴定。因此，该方法可以同时处理多种中药。此外，结果表明，该方法是从中药中选择性地提取目标化合物的有效方法。
