Lindner Herbert, Sarg Bettina, Helliger Wilfried
Department of Medical Chemistry and Biochemistry, University of Innsbruck, Innsbruck, Austria.
J Capill Electrophor Microchip Technol. 2003 May-Aug;8(3-4):59-67.
Histones are the most intensively studied group of basic nuclear proteins and are of great importance with regard to the organization of chromatin structure and control of gene activity. The complexity of this protein family places very high demands on the analytical methods applied. The advent of high-performance capillary electrophoresis provided a promising new tool for their separation, which is an essential prerequisite for studying the biological role of this protein family. Problems inherent to histone analysis due to their unique physical and chemical properties are reviewed, and the pros and cons of distinct strategies developed for CE separation of these proteins are discussed.
组蛋白是研究最为深入的一类碱性核蛋白，对于染色质结构的组织和基因活性的控制至关重要。该蛋白家族的复杂性对所应用的分析方法提出了很高的要求。高效毛细管电泳的出现为其分离提供了一种有前景的新工具，这是研究该蛋白家族生物学作用的必要前提。本文综述了由于组蛋白独特的物理和化学性质而在其分析过程中固有的问题，并讨论了为毛细管电泳分离这些蛋白而开发的不同策略的优缺点。
