Uryu Tsutomu, Yoshinaga Jun, Yanagisawa Yukio, Endo Masahiko, Takahashi Junichi
Institute of Environmental Studies, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan.
Anal Sci. 2003 Oct;19(10):1413-6. doi: 10.2116/analsci.19.1413.
Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was used to determine the Pb/Ca ratios in the enamel of deciduous incisors, a biomarker of in utero Pb exposure, using pelletized bone certified reference materials (CRMs) as calibrants. The detection limit for Pb by LA-ICP-MS was 11 microg kg(-1) demonstrating an adequate sensitivity for Pb in the teeth of unexposed individuals (0.1-10 mg kg(-1)). The precision for the Pb/Ca ratios in NIST SRM 1486 Bone Meal was 3.4%. The correlation between Pb/Ca ratios obtained by LA-ICP-MS and those obtained by a digestion method was highly significant. We found one point calibration by a CRM was applicable for the quantification of Pb in tooth enamel. This method will be valuable for the assessment of in utero Pb exposure levels.
采用激光烧蚀-电感耦合等离子体质谱法（LA-ICP-MS），以颗粒状骨标准参考物质（CRM）作为校准物，测定乳牙切牙牙釉质中的铅钙比，这是子宫内铅暴露的生物标志物。LA-ICP-MS对铅的检测限为11微克/千克，表明该方法对未暴露个体牙齿中的铅具有足够的灵敏度（0.1-10毫克/千克）。NIST SRM 1486骨粉中铅钙比的精密度为3.4%。LA-ICP-MS获得的铅钙比与消化法获得的铅钙比之间的相关性非常显著。我们发现通过CRM进行单点校准适用于牙釉质中铅的定量分析。该方法对于评估子宫内铅暴露水平具有重要价值。
