Ruso Juan M, González-Pérez Alfredo, Prieto Gerardo, Sarmiento Félix
Department of Applied Physics, Group of Biophysics and Interfaces, Faculty of Physics, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
Int J Biol Macromol. 2003 Nov;33(1-3):67-73. doi: 10.1016/s0141-8130(03)00068-0.
The interactions of a fluorinated surfactant, sodium perfluorooctanoate, with lysozyme, have been investigated by a combination of UV absorbance, electrical conductivity and dynamic light scattering to detect and to characterize the conformational transitions of lysozyme. By using difference spectroscopy, the transition was followed as a function of surfactant concentration, and the data were analyzed to obtain the Gibbs energy of the transition in water (DeltaGw(o)) and in a hydrophobic environment (DeltaGh(o)) for saturated protein-surfactant complexes. Electrical conductivity was used to determine the critical micelle concentration of the surfactant in the presence of different lysozyme concentration. From these results, the average number of surfactant monomer per protein molecule was calculated. Finally, dynamic light scattering show that only changes in the secondary structure of the protein can be observed.
通过紫外吸收、电导率和动态光散射相结合的方法，研究了全氟辛酸钠这种含氟表面活性剂与溶菌酶的相互作用，以检测和表征溶菌酶的构象转变。利用差示光谱法，跟踪了转变随表面活性剂浓度的变化情况，并对数据进行分析，以获得饱和蛋白质 - 表面活性剂复合物在水中（ΔGw(o)）和疏水环境中（ΔGh(o)）转变的吉布斯自由能。在不同溶菌酶浓度存在的情况下，用电导率来测定表面活性剂的临界胶束浓度。根据这些结果，计算出每个蛋白质分子上表面活性剂单体的平均数量。最后，动态光散射表明只能观察到蛋白质二级结构的变化。
