Peltonen K, Canella K, Dipple A
Chemistry of Carcinogenesis Laboratory, NCI-Frederick Cancer Research and Development Center, MD 21702.
J Chromatogr. 1992 Oct 16;623(2):247-54. doi: 10.1016/0021-9673(92)80364-z.
Chromatographic methods that allow the separation of adducts of purine nucleoside 3'-phosphates with the pure enantiomers of the anti-dihydrodiol epoxide of benzo[a]pyrene are developed. The optimization procedure includes evaluation of the effect of buffer molarity, the pH of the buffer, and the role of organic modifiers. The method can be utilized to prepare standards with known absolute configuration that can be further used in the Randerath 32P-postlabeling procedure.
开发了能够分离嘌呤核苷3'-磷酸与苯并[a]芘反式二氢二醇环氧化物纯对映体加合物的色谱方法。优化过程包括评估缓冲液摩尔浓度、缓冲液pH值以及有机改性剂的作用。该方法可用于制备具有已知绝对构型的标准品，这些标准品可进一步用于Randerath 32P后标记程序。
