Schwer C, Lottspeich F
Department of Protein Chemistry, Max-Planck-Institute for Biochemistry, Martinsried, Germany.
J Chromatogr. 1992 Oct 16;623(2):345-55. doi: 10.1016/0021-9673(92)80375-5.
The tiny injection volumes that are usually necessary to maintain the high efficiency of capillary zone electrophoresis present a major problem if only limited sample amounts are available. To increase the sample load, discontinuous buffer systems were developed that allow the on-column concentration of dilute samples. Injection volumes can be increased in this way by at least a factor of 30. These stacking systems were applied to the analysis of tryptic peptides, to the purity checking of high-performance liquid chromatographic fractions and for the micropreparative separation of peptides with subsequent amino acid sequence analysis.
若仅有有限量的样品可用，为维持毛细管区带电泳的高效性通常所需的微量进样体积便成为一个主要问题。为增加样品进样量，人们开发了不连续缓冲体系，该体系可实现柱上对稀释样品的浓缩。通过这种方式，进样体积可至少增大30倍。这些堆积系统被应用于胰蛋白酶解肽段的分析、高效液相色谱馏分的纯度检测以及肽段的微量制备分离及后续氨基酸序列分析。
