Baccari Gabriella Chieffi, Raucci Franca, Di Fiore Maria M, Monteforte Rossella
Dipartimento di Scienze della Vita, Seconda Università degli Studi di Napoli, Via Vivaldi, 43-81100 Caserta, Italy.
Microsc Res Tech. 2003 Dec 1;62(5):439-50. doi: 10.1002/jemt.10391.
The effect of nerve growth factor (NGF) on ontogenesis of frog mast cells was investigated in vivo by histochemical, morphometric, and ultrastructural analysis. Three groups of tadpoles at various stages of development were used. In the first group, the larvae received i.p. injections of 1 ng NGF/g; the second group received 10 ng NGF/g, while the control group received only the vehicle. The first recognizable mast cells arose symmetrically in the tongue at stage 26 of Witschi's standard table. At stages 26 and 29, the mast cell number in the NGF-injected tadpoles was significantly higher than the control group. From stage 29 onward, the mast cell number rapidly increased in all groups. No significant differences in mast cell number were observed between the control group and the NGF-injected groups at stages 31 and 33. Electron microscopy revealed that at metamorphic climax (stage 33), the mast cells in the NGF-treated groups were more mature than those in the control group. Therefore, nerve growth factor at early stages of tadpole development is likely to induce differentiation of mast cell precursors, while at later stages it is likely to induce maturation of immature mast cells. The close anatomical association between mast cells and perineurium, observed during nerve development, is intriguing. Already in the early stages of nerve development, the mast cells form a network around Schwann cell-axon complexes, together with the perineurial cells. At climax, the mast cells are located between the perineurial layers, suggesting that they may play a role in the tissue-nerve barrier of the perineurium. Nerve growth factor also seems to induce perineurial cell maturation.
通过组织化学、形态计量学和超微结构分析，在体内研究了神经生长因子（NGF）对青蛙肥大细胞个体发育的影响。使用了处于不同发育阶段的三组蝌蚪。第一组幼虫腹腔注射1 ng NGF/g；第二组注射10 ng NGF/g，而对照组仅注射溶剂。在维茨基标准表的第26阶段，首次识别出的肥大细胞对称地出现在舌头中。在第26和29阶段，注射NGF的蝌蚪中的肥大细胞数量明显高于对照组。从第29阶段开始，所有组中的肥大细胞数量迅速增加。在第31和33阶段，对照组和注射NGF的组之间在肥大细胞数量上未观察到显著差异。电子显微镜显示，在变态高峰期（第33阶段），NGF处理组中的肥大细胞比对照组中的更成熟。因此，蝌蚪发育早期的神经生长因子可能诱导肥大细胞前体的分化，而在后期它可能诱导未成熟肥大细胞的成熟。在神经发育过程中观察到的肥大细胞与神经束膜之间紧密的解剖学联系很有趣。在神经发育的早期阶段，肥大细胞就与神经束膜细胞一起在雪旺细胞 - 轴突复合体周围形成网络。在高峰期，肥大细胞位于神经束膜层之间，这表明它们可能在神经束膜的组织 - 神经屏障中起作用。神经生长因子似乎也能诱导神经束膜细胞成熟。
