Xiong Zuying, Huang Haichang, Li Jingzi, Wang Haiyan
Renal Division, Peking University First Hospital, Beijing, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2003 Oct;35(5):499-502.
To investigate peroxisome proliferator-activated receptor gamma (PPAR-gamma) expressions in patients with actively proliferating glomerulonephritis such as type IV lupus nephritis (LN) and cellular crescentic glomerulonephritis (RPGN).
All patients were divided into 4 groups as follows: RPGN (17 cases); LN type IV (15 cases); mild mesangial proliferative IgA nephropathy (IgAN, 7 cases) and minimal change disease (MCD, 10 cases). Clinical parameters, immunohisto-chemistry stain and in situ hybridization of renal biopsy specimens were performed.
Clinically, proteinuria and hematouria were increased and Ccr were decreased in LN and RPGN patients, and increased ESR and decreased complement C3 were found in group LN. Active index of renal specimens were significantly higher in LN and RPGN groups than in IgAN and MCD groups. Renal specimens of MCD patients showed no positive PPAR-gamma staining in all sections; little immunoreactivity was detected in sections of glomerular, tubular and interstitial cells from IgAN patients. Glomerular positive staining of PPAR-gamma in renal sections in LN and RPGN patients[(3.3 +/- 1.8) and (2.8 +/- 1.2) cells per section of glomerulus, respectively] were significantly increased compared with that in IgAN patients [(0.7 +/- 0.5) cells per section of glomerulus]. Similarly, tubular positive staining of PPAR-gamma in LN and RPGN patients (27.38 +/- 12.46, 23.36 +/- 10.55) were also elevated compared with that in IgAN and MCD patients(6.51 +/- 3.49, 1.72 +/- 0.31). The relevance assay results showed positive relationship between active index and glomerular or tubular PPAR-gamma immunohisto-chemistry staining cell numbers (0.478, P < 0.01; 0.513, P < 0.01). Tubular PPAR-gamma mRNA expression in LN and RPGN patients was upregulated by in situ hybridization.
Our results demonstrate in vivo that PPAR-gamma expression was increased in active glomerulonephritis patients accompanied with clinical and pathological active inflammation. These data suggest that increase of PPAR-gamma expression in renal cells may play an important role in response to inflammatory stress.
研究过氧化物酶体增殖物激活受体γ（PPAR-γ）在活动性增殖性肾小球肾炎患者中的表达，如IV型狼疮性肾炎（LN）和细胞性新月体性肾小球肾炎（RPGN）。
将所有患者分为以下4组：RPGN组（17例）；IV型LN组（15例）；轻度系膜增生性IgA肾病（IgAN，7例）和微小病变病（MCD，10例）。对肾活检标本进行临床参数、免疫组织化学染色和原位杂交检测。
临床上，LN和RPGN患者蛋白尿和血尿增加，肌酐清除率降低，LN组血沉升高、补体C3降低。LN和RPGN组肾标本的活动指数显著高于IgAN和MCD组。MCD患者的肾标本在所有切片中均未显示PPAR-γ染色阳性；IgAN患者的肾小球、肾小管和间质细胞切片中检测到的免疫反应性较弱。LN和RPGN患者肾切片中PPAR-γ的肾小球阳性染色[分别为每肾小球切片（3.3±1.8）个细胞和（2.8±1.2）个细胞]与IgAN患者[每肾小球切片（0.7±0.5）个细胞]相比显著增加。同样，LN和RPGN患者肾小管PPAR-γ的阳性染色（27.38±12.46，23.36±10.55）也高于IgAN和MCD患者（6.51±3.49，1.72±0.31）。相关性分析结果显示活动指数与肾小球或肾小管PPAR-γ免疫组织化学染色细胞数之间呈正相关（0.478，P<0.01；0.513，P<0.01）。通过原位杂交检测发现LN和RPGN患者肾小管PPAR-γ mRNA表达上调。
我们的结果在体内证明，活动性肾小球肾炎患者PPAR-γ表达增加，伴有临床和病理活动性炎症。这些数据表明肾细胞中PPAR-γ表达的增加可能在应对炎症应激中起重要作用。
