Valueva T A, Revina T A, Gvozdeva E L, Gerasimova N G, Ozeretskovskaia O L
Bakh Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071 Russia.
Bioorg Khim. 2003 Sep-Oct;29(5):499-504. doi: 10.1023/a:1026053626260.
Mechanical damage or infection of potatoes with Phytophthora infestans caused an accumulation of only serine protease inhibitors in exudates of potato tubers. Among them, proteins prevailed that are structurally similar to those present in healthy tubers: a 22-kDa trypsin inhibitor, a 21-kDa serine protease inhibitor consisting of two polypeptide chains, and a 8-kDa potato chymotrypsin I inhibitor produced de novo. The accumulated proteins inhibited the growth of hyphae and germination of zoospores of P. infestans. Treatment with elicitors, jasmonic and arachidonic acids, intensified the accumulation of these inhibitors in tubers in response to the wound stress, whereas salicylic acid blocked this process. These results suggest that the lipoxygenase metabolism plays a substantial role in signal transduction of the protective system of resting potato tubers.
马铃薯受到机械损伤或被致病疫霉感染后，其块茎渗出物中只会积累丝氨酸蛋白酶抑制剂。其中，占主导地位的蛋白质在结构上与健康块茎中的蛋白质相似：一种22 kDa的胰蛋白酶抑制剂、一种由两条多肽链组成的21 kDa丝氨酸蛋白酶抑制剂，以及一种新产生的8 kDa马铃薯胰凝乳蛋白酶I抑制剂。积累的蛋白质抑制了致病疫霉的菌丝生长和游动孢子萌发。用茉莉酸和花生四烯酸等诱导子处理，会增强这些抑制剂在块茎中因伤口胁迫而产生的积累，而水杨酸则会阻断这一过程。这些结果表明，脂氧合酶代谢在休眠马铃薯块茎保护系统的信号转导中起着重要作用。
