Zhu M, Wesley I V, Nannapaneni R, Cox M, Mendonca A, Johnson M G, Ahn D U
Pre-Harvest Food Safety and Enteric Diseases Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa 50010, USA.
Poult Sci. 2003 Oct;82(10):1559-64. doi: 10.1093/ps/82.10.1559.
The current study was designed to determine if dietary vitamin E influenced either the gut clearance or levels of peripheral blood CD4+ and CD8+ T lymphocytes in adult turkeys experimentally infected with Listeria monocytogenes. Turkeys were fed vitamin E (0, 100, or 200 IU) from day of hatch to time of necropsy. After 6 wk on the experimental diet, turkeys were orally inoculated with L. monocytogenes (approximately 10(9) cfu). To monitor infection status, cloacal swabs were taken on selected days post-inoculation (DPI). At necropsy, samples of viscera, including liver, spleen, cecum, duodenum, ileum, and colon were collected and cultured for L. monocytogenes. In experiments 1 and 2, recovery of L. monocytogenes from cloacal swabs, tissues, and intestines from turkeys fed vitamin E was generally lower than that from turkeys fed the control diet, although these differences were not statistically significant. When data from both trials were combined, L. monocytogenes was cultured less frequently from cloacal swabs of the vitamin E-treated group (200 IU) on 2 and 3 DPI, when compared to controls (0 IU, P < 0.01). There were no changes in virulence characteristics of L. monocytogenes cells, as measured by in vitro killing of Ped-2E9 cells, recovered from cloacal swabs or tissues of experimentally infected turkeys fed the control or a vitamin E treatment diet. Flow cytometric analysis indicated that CD4+ and CD8+ peripheral blood T lymphocytes were elevated at 6 and 8 DPI in infected turkeys given 200 IU vitamin E.
本研究旨在确定日粮维生素E是否会影响经实验感染单核细胞增生李斯特菌的成年火鸡的肠道清除率或外周血CD4+和CD8+ T淋巴细胞水平。从孵化日至尸检时，给火鸡饲喂维生素E（0、100或200 IU）。在实验日粮喂养6周后，给火鸡口服接种单核细胞增生李斯特菌（约10⁹ cfu）。为监测感染状况，在接种后选定的日子采集泄殖腔拭子。尸检时，收集包括肝脏、脾脏、盲肠、十二指肠、回肠和结肠在内的内脏样本，并培养单核细胞增生李斯特菌。在实验1和2中，从饲喂维生素E的火鸡的泄殖腔拭子、组织和肠道中回收的单核细胞增生李斯特菌通常低于饲喂对照日粮的火鸡，尽管这些差异无统计学意义。当将两项试验的数据合并时，与对照组（0 IU，P < 0.01）相比，在接种后第2天和第3天，从维生素E处理组（200 IU）的泄殖腔拭子中培养出单核细胞增生李斯特菌的频率较低。从饲喂对照或维生素E处理日粮的实验感染火鸡的泄殖腔拭子或组织中回收的单核细胞增生李斯特菌细胞，通过体外杀伤Ped-2E9细胞测定，其毒力特征没有变化。流式细胞术分析表明，在感染后第6天和第8天，给予200 IU维生素E的感染火鸡外周血CD4+和CD8+ T淋巴细胞升高。
