Subauste C S, Dawson L, Remington J S
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301.
J Exp Med. 1992 Dec 1;176(6):1511-9. doi: 10.1084/jem.176.6.1511.
Experiments were conducted to determine whether human lymphokine-activated killer (LAK) cells are cytotoxic against cells infected with Toxoplasma gondii. Nylon wool nonadherent (NWNA) peripheral blood lymphocytes, as well as purified natural killer cell (NK) (CD3- CD16+ CD56+) and T (CD3+ CD16- CD56-) cells obtained from five healthy T. gondii seronegative volunteers exhibited minimal cytotoxic activity against T. gondii-infected cells. When standard LAK (S-LAK) cell preparations were induced by incubation of NWNA cells with recombinant interleukin 2, induction of remarkable cytotoxic activity against T. gondii-infected cells. When standard in LAK cell preparations from each of the volunteers. The phenotype of the LAK precursor and effector cells varied depending on the target cell used. Whereas the precursor and the effector cells of most of the LAK activity against K562 and Daudi cells were cells with NK phenotype, when T. gondii-infected cells were used as targets, both cells with NK and T cell phenotypes were precursors and effectors of the lysis. When cytotoxic activity of S-LAK cells was compared with the activity of adherent LAK (A-LAK) cells, A-LAK cells displayed higher cytotoxic activity against T. gondii-infected cells, as well as against K562 and Daudi cells. Cold target inhibition experiments suggested that there is a subset of LAK effector cells capable of lysing both T. gondii-infected cells and Daudi cells, whereas other subsets preferentially or exclusively lyse one of these target cells.
进行实验以确定人类淋巴因子激活的杀伤（LAK）细胞是否对感染弓形虫的细胞具有细胞毒性。从五名健康的弓形虫血清阴性志愿者中获得的尼龙毛非黏附（NWNA）外周血淋巴细胞，以及纯化的自然杀伤细胞（NK）（CD3-CD16+CD56+）和T细胞（CD3+CD16-CD56-）对弓形虫感染的细胞表现出最小的细胞毒性活性。当通过将NWNA细胞与重组白细胞介素2孵育来诱导标准LAK（S-LAK）细胞制剂时，可诱导出对弓形虫感染细胞的显著细胞毒性活性。当从每位志愿者的LAK细胞制剂中进行标准化时。LAK前体细胞和效应细胞的表型因所用靶细胞而异。虽然针对K562和Daudi细胞的大多数LAK活性的前体细胞和效应细胞是具有NK表型的细胞，但当使用弓形虫感染的细胞作为靶标时，具有NK和T细胞表型的细胞都是裂解的前体细胞和效应细胞。当比较S-LAK细胞的细胞毒性活性与黏附性LAK（A-LAK）细胞的活性时，A-LAK细胞对弓形虫感染的细胞以及对K562和Daudi细胞表现出更高的细胞毒性活性。冷靶抑制实验表明，存在一部分LAK效应细胞能够裂解弓形虫感染的细胞和Daudi细胞，而其他部分则优先或仅裂解这些靶细胞中的一种。
