用于诊断弓形虫感染的直接凝集试验：提高敏感性和特异性的方法
Direct agglutination test for diagnosis of Toxoplasma infection: method for increasing sensitivity and specificity.
作者信息
Desmonts G, Remington J S
出版信息
J Clin Microbiol. 1980 Jun;11(6):562-8. doi: 10.1128/jcm.11.6.562-568.1980.
Abstract
A method that increases the sensitivity and specificity of the direct agglutination (AG) test for diagnosis of Toxoplasma gondii infection is described. Qualitative results in the Sabin-Feldman dye test (DT) and AG test were in excellent agreement (98%). Differences in titers between these two tests often related to the length of time the individual was infected. The AG test titer was most often lower than the DT titer during acute (recent) infection; the AG test titer was most often higher than the DT titer in older or chronic infection. If the AG test antigen described here can be made available, the AG test would be ideal for use as a screening test and would provide a simple and inexpensive means for the surveillance of seronegative women during pregnancy and for detection of seroconversions.
摘要
本文描述了一种提高用于诊断弓形虫感染的直接凝集（AG）试验的灵敏度和特异性的方法。Sabin-Feldman染色试验（DT）和AG试验的定性结果一致性极佳（98%）。这两种试验之间滴度的差异通常与个体感染的时间长短有关。在急性（近期）感染期间，AG试验滴度最常低于DT试验滴度；在较久或慢性感染中，AG试验滴度最常高于DT试验滴度。如果本文所述的AG试验抗原能够得到应用，那么AG试验将是用作筛查试验的理想选择，并将为孕期血清阴性女性的监测以及血清转化的检测提供一种简单且廉价的方法。
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