利用编码铜/锌胞质超氧化物歧化酶、含信号肽超氧化物歧化酶和谷胱甘肽过氧化物酶的基因进行DNA疫苗接种来预防曼氏血吸虫感染。

Protection against Schistosoma mansoni utilizing DNA vaccination with genes encoding Cu/Zn cytosolic superoxide dismutase, signal peptide-containing superoxide dismutase and glutathione peroxidase enzymes.

作者信息

Shalaby Kamal A, Yin Lei, Thakur Arvind, Christen Linda, Niles Edward G, LoVerde Philip T

机构信息

Department of Microbiology, and Immunology, School of Medicine and Biomedical Sciences, State University of New York, 138 Farber Hall, Buffalo, NY 14214, USA.

出版信息

Vaccine. 2003 Dec 8;22(1):130-6. doi: 10.1016/s0264-410x(03)00535-8.

DOI:10.1016/s0264-410x(03)00535-8

PMID:14604580

Abstract

Protection against Schistosoma mansoni infection in C57BL/6 female mice was evaluated by two DNA vaccination strategies. Mice were either vaccinated by intramuscular injection with pcDNAI/Amp constructs encoding either Cu/Zn cytosolic superoxide dismutase (CT-SOD), signal peptide-containing SOD (SP-SOD), glutathione peroxidase (GPX(bb)) or a mutated form of GPX (GPX(m)), or primed with naked DNA constructs and boosted with recombinant vaccinia virus (RVV) containing the same genes. Animals were then challenged with S. mansoni and the level of protection was assessed as the reduction in worm burden. CT-SOD showed significant levels of protection compared to the control group, ranging between 44 and 60%, while SP-SOD exhibited from 22 to 45%. GPX(bb) showed levels of protection (23-55%) higher than GPX(m) (25-34%). The prime-boost strategy gave the same results as naked DNA or recombinant vaccinia virus alone except in the case of GPX, where the protection was 85%.

摘要

通过两种DNA疫苗接种策略评估了C57BL/6雌性小鼠对曼氏血吸虫感染的抵抗力。小鼠通过肌肉注射接种编码铜/锌胞质超氧化物歧化酶（CT-SOD）、含信号肽的SOD（SP-SOD）、谷胱甘肽过氧化物酶（GPX(bb)）或GPX的突变形式（GPX(m)）的pcDNAI/Amp构建体进行疫苗接种，或者先用裸DNA构建体进行初免，再用含有相同基因的重组痘苗病毒（RVV）进行加强免疫。然后用曼氏血吸虫对动物进行攻击，并将保护水平评估为虫负荷的降低。与对照组相比，CT-SOD显示出显著的保护水平，范围在44%至60%之间，而SP-SOD的保护水平在22%至45%之间。GPX(bb)的保护水平（23%至55%）高于GPX(m)（25%至34%）。初免-加强策略产生的结果与单独使用裸DNA或重组痘苗病毒相同，但GPX的情况除外，其保护率为85%。