经口服给予眼镜蛇毒的兔子血清可抑制小鼠体内植入的肝癌细胞生长。
Serum from rabbit orally administered cobra venom inhibits growth of implanted hepatocellular carcinoma cells in mice.
作者信息
Sun Peng, Ren Xian-Da, Zhang Hai-Wei, Li Xiao-Hong, Cai Shao-Hui, Ye Kai-He, Li Xiao-Kun
机构信息
Department of Clinical Pharmacology, Pharmacy College, Jinan University, Guangzhou 510632, Guangdong Province, China.
出版信息
World J Gastroenterol. 2003 Nov;9(11):2441-4. doi: 10.3748/wjg.v9.i11.2441.
AIM
To investigate the inhibitory effect of serum preparation from rabbits orally administered cobra venom (SRCV) on implanted hepatocellular carcinoma (HCC) cells in mice.
METHODS
An HCC cell line, HepA, was injected into mice to prepare implanted tumors. The animals (n=30) were divided randomly into SRCV, 5-fluorouracil (5-FU), and distilled water (control) groups. From the second day after transplantation, 20 mg/kg 5-FU was administered intraperitoneally once a day for 9 days. SRCV (1,000 mg/kg) or distilled water (0.2 mL) was given by gastrogavage. Tumor growth inhibition was described by the inhibitory rate (IR). Apoptosis was detected by transmission electron microscopy (TEM), flow cytometry (FCM), and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL). Student's t-test was performed for statistical analysis.
RESULTS
The tumor growth was inhibited markedly by SRCV treatment compared to that in the control group (P<0.01). The treatment resulted in a significant increase in the apoptotic rate of cancer cells by the factors of 10.5+/-2.4 % and 20.65+/-3.2 % as demonstrated through TUNEL and FCM assays, respectively (P<0.01). The apoptotic cells were also identified by characteristic ultrastructural features.
CONCLUSION
SRCV can inhibit the growth of implanted HepA cells in mice, and the apoptosis rate appears to elevate during the process.
目的
研究经口给予眼镜蛇毒的兔血清制剂（SRCV）对小鼠移植性肝癌（HCC）细胞的抑制作用。
方法
将肝癌细胞系HepA注入小鼠体内制备移植瘤。将30只动物随机分为SRCV组、5-氟尿嘧啶（5-FU）组和蒸馏水（对照组）。移植后第二天起，每天腹腔注射20 mg/kg 5-FU，共9天。通过灌胃给予SRCV（1000 mg/kg）或蒸馏水（0.2 mL）。用抑制率（IR）描述肿瘤生长抑制情况。通过透射电子显微镜（TEM）、流式细胞术（FCM）和末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记（TUNEL）检测细胞凋亡。采用Student's t检验进行统计学分析。
结果
与对照组相比，SRCV治疗显著抑制了肿瘤生长（P<0.01）。TUNEL和FCM检测分别显示，该治疗使癌细胞凋亡率显著提高，分别为10.5±2.4%和20.65±3.2%（P<0.01）。凋亡细胞也通过特征性超微结构特征得以鉴定。
结论
SRCV可抑制小鼠体内移植的HepA细胞生长，且在此过程中凋亡率似乎升高。
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