Saito K, Yoshida K, Kohno N, Nakanuma Y
Second Department of Pathology, Kanazawa University School of Medicine.
Nihon Shokakibyo Gakkai Zasshi. 1992 Nov;89(11):2693-9.
We established a new method of purification and culture of mouse gallbladder epithelial cells. In primary culture, mouse gallbladder tissue was cultured on collagen gel using microexplant culture method; the epithelial cells spread in a single sheet from the microexplant toward the periphery on the collagen gel. Tiny fragment of the collagen gel containing the border of epithelial cell layer was retransplanted onto the another collagen gel for secondary culture. This retransplantation allowed the proliferation of the epithelial cells without mesenchymal contamination, which spread on the collagen gel at the rate of 0.29 +/- 0.06 mm per day for more than two weeks. The cultured epithelial cells showed cuboid or columnar shape with focal mucus production, which is morphologically and functionally similar to the in vivo epithelial cells. This suggests that this in vitro culture method can be used to pathophysiological studies of biliary epithelial cells as well as for the method of purification of the epithelial cells.
我们建立了一种新的小鼠胆囊上皮细胞纯化和培养方法。在原代培养中，采用微植块培养法将小鼠胆囊组织培养在胶原凝胶上；上皮细胞从微植块在胶原凝胶上呈单层向周边扩散。将含有上皮细胞层边界的胶原凝胶小片段重新移植到另一块胶原凝胶上进行传代培养。这种重新移植使得上皮细胞能够增殖而无间充质污染，上皮细胞在胶原凝胶上以每天0.29±0.06毫米的速度扩散超过两周。培养的上皮细胞呈立方形或柱状，有局灶性黏液分泌，在形态和功能上与体内上皮细胞相似。这表明这种体外培养方法可用于胆道上皮细胞的病理生理学研究以及上皮细胞的纯化方法。
