Shearer William T, Rosenblatt Howard M, Gelman Rebecca S, Oyomopito Rebecca, Plaeger Susan, Stiehm E Richard, Wara Diane W, Douglas Steven D, Luzuriaga Katherine, McFarland Elizabeth J, Yogev Ram, Rathore Mobeen H, Levy Wende, Graham Bobbie L, Spector Stephen A
Baylor College of Medicine, Houston, Tex, USA.
J Allergy Clin Immunol. 2003 Nov;112(5):973-80. doi: 10.1016/j.jaci.2003.07.003.
Peripheral blood lymphocyte subsets need to be determined in a large, urban, minority-predominant cohort of healthy children to serve as suitable control subjects for the interpretation of the appearance of these cells in several disease conditions, notably pediatric HIV-1 infection.
We sought to determine the distribution of lymphocyte subsets in healthy urban-dwelling infants, children, and adolescents in the United States.
Lymphocyte subsets were determined by means of 3-color flow cytometry in a cross-sectional study of 807 HIV-unexposed children from birth through 18 years of age.
Cell-surface marker analysis demonstrated that age was an extremely important variable in 24 lymphocyte subset distributions measured as percentages or absolute counts--eg, the CD4 (helper) T cell, CD8 (cytotoxic) T cell, CD19 B cell, CD4CD45RACD62L (naive helper) T cell, CD3CD4CD45RO (memory helper) T cell, CD8HLA-DRCD38 (activated cytotoxic) T cell, and CD8CD28 (activation primed cytotoxic) T cell. The testing laboratory proved to be an important variable, indicating the need for using the same laboratory or group of laboratories to assay an individual's blood over time and to assay control and ill or treated populations. Sex and race-ethnicity were much less important.
The results of this study provide a control population for assessment of the effects of HIV infection on the normal development and distribution of lymphocyte subsets in children of both sexes, all races, and all ethnic backgrounds from birth through 18 years of age in an urban population. This study's findings will also prove invaluable in interpreting the immune changes in children with many other chronic diseases, such as primary immunodeficiency, malignancy, rheumatoid arthritis, and asthma.
需要在一个以少数族裔为主的大型城市健康儿童队列中确定外周血淋巴细胞亚群，以便作为合适的对照对象，用于解释这些细胞在多种疾病状态下的表现，尤其是儿科HIV-1感染。
我们试图确定美国城市健康婴幼儿、儿童及青少年中淋巴细胞亚群的分布情况。
在一项横断面研究中，采用三色流式细胞术对807名未感染HIV的儿童（从出生至18岁）进行淋巴细胞亚群测定。
细胞表面标志物分析表明，年龄是24种淋巴细胞亚群分布的一个极其重要的变量，这些亚群以百分比或绝对计数来衡量，例如CD4（辅助性）T细胞、CD8（细胞毒性）T细胞、CD19 B细胞、CD4CD45RACD62L（幼稚辅助性）T细胞、CD3CD4CD45RO（记忆辅助性）T细胞、CD8HLA-DRCD38（活化细胞毒性）T细胞以及CD8CD28（活化预致敏细胞毒性）T细胞。检测实验室被证明是一个重要变量，这表明需要使用同一实验室或一组实验室来长期检测个体血液，并检测对照人群以及患病或接受治疗的人群。性别和种族-民族的重要性则小得多。
本研究结果提供了一个对照人群，用于评估HIV感染对城市人群中从出生至18岁的所有种族、所有民族背景的男女儿童淋巴细胞亚群正常发育和分布的影响。本研究的发现对于解释患有许多其他慢性疾病（如原发性免疫缺陷、恶性肿瘤、类风湿性关节炎和哮喘）的儿童的免疫变化也将具有巨大价值。
