一种双链RNA真菌病毒赋予灰葡萄孢菌与低毒力相关的特性。

A double-stranded RNA mycovirus confers hypovirulence-associated traits to Botrytis cinerea.

作者信息

Castro Miguel, Kramer Kurt, Valdivia Lorena, Ortiz Sylvia, Castillo Antonio

机构信息

Laboratorio de Virología de Hongos, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile.

出版信息

FEMS Microbiol Lett. 2003 Nov 7;228(1):87-91. doi: 10.1016/S0378-1097(03)00755-9.

DOI:10.1016/S0378-1097(03)00755-9

PMID:14612241

Abstract

Botrytis cinerea CCg425 contains a 33-nm isometric mycovirus whose genome is a 6.8-kb double-stranded RNA (dsRNA) molecule. Virulence bioassays, performed by direct plug mycelial inoculation on bean plant leaves, showed that B. cinerea CCg425 displays less fungal aggressivity than B. cinerea CKg54, a virulent fungal strain that is not infected by dsRNA mycoviruses. B. cinerea CCg425 also showed lower laccase activity and conidiation rate than B. cinerea CKg54. Furthermore, infection of B. cinerea CKg54 with viral particles purified from B. cinerea CCg425 resulted in diminished virulence of the infected fungus. Collectively, our results indicate that mycovirus infection confers hypovirulence to the fungal host.

摘要

灰葡萄孢CCg425含有一种33纳米的等轴真菌病毒，其基因组是一个6.8千碱基的双链RNA（dsRNA）分子。通过直接在菜豆叶片上接种菌丝块进行的毒力生物测定表明，与未被dsRNA真菌病毒感染的强毒真菌菌株灰葡萄孢CKg54相比，灰葡萄孢CCg425的真菌侵袭力较弱。灰葡萄孢CCg425的漆酶活性和产孢率也低于灰葡萄孢CKg54。此外，用从灰葡萄孢CCg425中纯化的病毒粒子感染灰葡萄孢CKg54，导致被感染真菌的毒力减弱。总体而言，我们的结果表明，真菌病毒感染使真菌宿主产生低毒力。