Chen Meng, Zamora Paul O, Peña Louis, Som Prantika, Osaki Shigemasa
Biosurface Engineering Technologies, Inc., 387 Technology Drive, College Park, Maryland 20742-3371, USA.
J Biomed Mater Res A. 2003 Dec 1;67(3):994-1000. doi: 10.1002/jbm.a.10115.
Stainless steel treated with a mixed gas plasma of NH(3) plus O(2) had chemical and biologic characteristics distinct from untreated stainless steel or stainless steel treated with NH(3) or O(2) plasmas used separately. NH(3)/O(2) plasmas deposited nitrogen as both -CN (organic) and -NO (nitrate, nitrite)--materials not found on untreated stainless steel--and the contact angle changed from 44 degrees to 23 degrees. Treatment of stainless steel (and titanium) resulted in surfaces with enhanced resistance to platelet and leukocyte attachment. A gas plasma of N(2)O/O(2) also was found to reduce platelet and leukocyte attachment, suggesting that these properties may be common to surfaces coated with oxynitrites (nitrides). Upon subcutaneous implantation, no inflammation, hemolysis, or untoward thrombosis was noted in the tissue surrounding the wafers treated with the NH(3)/O(2) plasmas, although the cellular density was considerably reduced by 2 weeks after implant. Collectively, the results suggest that NH(3)/O(2) plasmas impart a unique character to stainless steel that may be useful in the construction of medical devices.
用NH₃加O₂的混合气体等离子体处理的不锈钢具有与未处理的不锈钢或分别用NH₃或O₂等离子体处理的不锈钢不同的化学和生物学特性。NH₃/O₂等离子体沉积的氮既有-CN（有机）形式又有-NO（硝酸盐、亚硝酸盐）形式——这些物质在未处理的不锈钢上未发现——并且接触角从44度变为23度。对不锈钢（和钛）的处理导致表面对血小板和白细胞附着的抵抗力增强。还发现N₂O/O₂气体等离子体可减少血小板和白细胞附着，这表明这些特性可能是涂有氮氧化物（氮化物）的表面所共有的。皮下植入后，在用NH₃/O₂等离子体处理的晶片周围组织中未观察到炎症、溶血或不良血栓形成，尽管植入后2周细胞密度显著降低。总体而言，结果表明NH₃/O₂等离子体赋予不锈钢独特的特性，这可能在医疗设备的制造中有用。
