Ozturk Hayrettin, Buyukbayram Huseyin, Ozdemir Enver, Ketani Aydin, Gurel Ahmet, Onen Abdurrahman, Otçu Selcuk
Department of Paediatric Surgery, Dicle University, Medical School, Diyarbakir, Turkey.
J Pediatr Surg. 2003 Nov;38(11):1621-7. doi: 10.1016/s0022-3468(03)00573-6.
BACKGROUND/PURPOSE: The aim of this study was to determine the effects of nitric oxide (NO) on the expression of adhesion molecules in the early course of testicular I-R injury in rats.
Forty male Sprague-Dawley rats were separated into 4 groups, each containing 10 rats. A sham operation was performed in group 1 (control). In group 2 (I-R), after 6 hours of unilateral testicular torsion, 1-hour detorsion of the testis was performed. In group 3 (I-R/L-NAME), after performing the same surgical procedures as in group II, L-NAME was given for 30 minutes. In group 4 (I-R/Mol), after performing the same surgical procedure (torsion and detorsion) as in group II, molsidomine, an NO donor, was infused for 30 minutes. Then, ipsilateral orchiectomies were performed to measure the tissue levels of malondialdehyde (MDA) and NO and to make histologic examination.
MDA values and the testicular injury score decreased and NO values increased in the I-R/Mol-treated group compared with other experimental groups. The tenascin expression in the interstitial space and basement membrane of the tubuli seminiferi were milder in the I-R/Mol group compared with that of the I-R and the I-R/L-NAME. The acrosomes of the spermatids in I-R and I-R/L-NAME groups were stained mildly by lectin. In the I-R and I-R/L-NAME groups, the interstitial spaces, basement membrane of the tubuli seminiferi, and sertoli and germinal cells in testicular tissue were stained intensely by ICAM-1.
The expression of adhesion molecules such as tenascin, lectin, and ICAM-1 in the torted testicular tissue may be a pathophysiologic sign of inflammation. NO regulates adhesion molecules expression.
背景/目的：本研究旨在确定一氧化氮（NO）对大鼠睾丸缺血再灌注（I-R）损伤早期黏附分子表达的影响。
40只雄性Sprague-Dawley大鼠分为4组，每组10只。第1组（对照组）行假手术。第2组（I-R组），单侧睾丸扭转6小时后，进行1小时的睾丸松解。第3组（I-R/L-NAME组），在进行与第2组相同的手术操作后，给予L-NAME 30分钟。第4组（I-R/Mol组），在进行与第2组相同的手术操作（扭转和解旋）后，输注NO供体吗多明30分钟。然后，进行同侧睾丸切除术，以测量丙二醛（MDA）和NO的组织水平并进行组织学检查。
与其他实验组相比，I-R/Mol治疗组的MDA值和睾丸损伤评分降低，NO值升高。与I-R组和I-R/L-NAME组相比，I-R/Mol组曲细精管间质和基底膜中的腱生蛋白表达较轻。I-R组和I-R/L-NAME组中，精子细胞的顶体被凝集素轻度染色。在I-R组和I-R/L-NAME组中，睾丸组织中的间质、曲细精管基底膜以及支持细胞和生殖细胞被细胞间黏附分子-1（ICAM-1）强烈染色。
扭转睾丸组织中腱生蛋白、凝集素和ICAM-1等黏附分子的表达可能是炎症的病理生理标志。NO调节黏附分子的表达。
