Ozkan K U, Boran C, Kilinç M, Garipardiç M, Kurutaş E B
Department of Pediatric Surgery, Kahramanmaraş Sütçümam University, Medical Faculty, Kahramanmaraş, Turkey.
J Pediatr Surg. 2004 Jan;39(1):91-5. doi: 10.1016/j.jpedsurg.2003.09.013.
BACKGROUND/PURPOSE: The aim of this study is to investigate the effect of zinc aspartate (ZA) pretreatment on ischemia-reperfusion (I/R) injury and blood and tissue antioxidant enzyme activity early after unilateral testicular torsion-detorsion (T/D).
Forty prepubertal male Sprague-Dawley rats (weight 160 to 220 g) were divided into 4 groups each containing 10 rats. Surgery was conducted under intraperitoneal 1-shot ketamine (50 mg/kg) anesthesia. The scrotum was entered through a midline incision. Rotating the left testis 720 degrees in a clockwise direction was the model of the testicular torsion. Group 1 was for the basal values. Group 2 had 4 hours T/D. Group 3 also had 4 hours T/D and pretreated with 50 mg/kg intraperitoneal ZA injection half an hour before detorsion. Group 4 was designed as a sham group. In the Group 2 and Group 3, the tunica vaginalis was opened, and left testicles were rotated clockwise 720 degrees and maintained in this torsion position by fixing with a silk suture to the scrotal wall. The scrotum was closed and 4 hours later reentered for testicular detorsion. After spermatic cord detorsion, the scrotum was closed. At the end of 4 hours detorsion period, bilateral orchiectomies were performed, and 5-mL intracardiac blood samples were taken. Blood and tissue superoxide dismutase (SOD), catalase (CAT) activities, and malondialdehyde (MDA) levels were measured, and histopathologic examination was performed.
Group 2 and group 3 had decreased blood SOD and CAT activities and elevated MDA levels indicating I/R injury. The 2 groups were also different from each other for these parameters reflecting the beneficial effect of ZA pretreatment (P <.05). The decreased ipsilateral tissue SOD and CAT activities in group 2 were different from the other groups including group 3 (P <.05). Ipsilateral tissue MDA levels of both group 2 and group 3 were elevated. Group 2 had higher values than group 3 (P <.05). In addition, specimens from group 2 had a significantly greater histologic injury than group 3 (P <.05). These findings were also supporting the beneficial effect of ZA pretreatment. All measurements of contralateral tests were similar to the basal values for all groups (P >.05).
ZA pretreatment reduces I/R injury by its antioxidant effects after unilateral testicular T/D and affects the antioxidant enzyme systems.
背景/目的：本研究旨在探讨天冬氨酸锌（ZA）预处理对单侧睾丸扭转-复位（T/D）后早期缺血再灌注（I/R）损伤以及血液和组织抗氧化酶活性的影响。
40只青春期前雄性Sprague-Dawley大鼠（体重160至220克）分为4组，每组10只。手术在腹腔注射一次性氯胺酮（50毫克/千克）麻醉下进行。经阴囊中线切口进入阴囊。顺时针旋转左侧睾丸720度作为睾丸扭转模型。第1组为基础值组。第2组进行4小时的T/D。第3组也进行4小时的T/D，并在复位前半小时腹腔注射50毫克/千克的ZA进行预处理。第4组设计为假手术组。在第2组和第3组中，打开鞘膜，将左侧睾丸顺时针旋转720度，并用丝线缝合固定于阴囊壁以维持此扭转位置。关闭阴囊，4小时后再次打开进行睾丸复位。精索复位后，关闭阴囊。在扭转4小时结束时，进行双侧睾丸切除术，并采集5毫升心脏内血液样本。检测血液和组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性以及丙二醛（MDA）水平，并进行组织病理学检查。
第2组和第3组血液中SOD和CAT活性降低，MDA水平升高，表明存在I/R损伤。这两组在这些反映ZA预处理有益作用的参数上也彼此不同（P<.05）。第2组同侧组织中SOD和CAT活性降低，与包括第3组在内的其他组不同（P<.05）。第2组和第3组同侧组织MDA水平均升高。第2组的值高于第3组（P<.05）。此外，第2组的标本组织学损伤明显大于第3组（P<.05）。这些发现也支持了ZA预处理的有益作用。所有组对侧睾丸的所有测量值均与基础值相似（P>.05）。
ZA预处理通过其抗氧化作用减轻单侧睾丸T/D后的I/R损伤，并影响抗氧化酶系统。
