用于基因诊断的基于自组装单分子层的表面增强拉曼散射基底。

Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics.

作者信息

Culha Mustafa, Stokes David, Allain Leonardo R, Vo-Dinh Tuan

机构信息

Advanced Biomedical Science and Technology Group, Oak Ridge National Laboratory, Bethel Valley Road, MS-6101 PO Box 2008, Oak Ridge, Tennessee 37831-6101, USA.

出版信息

Anal Chem. 2003 Nov 15;75(22):6196-201. doi: 10.1021/ac0346003.

DOI:10.1021/ac0346003

PMID:14616001

Abstract

The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH-(CH(2))(6)-ssDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microscopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.

摘要

描述了用于癌症基因检测的表面增强拉曼散射（SERS）活性基底的开发。该检测方法使用拉曼活性染料标记的DNA基因探针、自组装单分子层和纳米结构金属基底作为SERS活性平台。通过原子力显微镜对在银表面形成的巯基己烷标记的单链DNA（SH-(CH(2))(6)-ssDNA）/6-巯基-1-己醇体系进行了表征。本研究中开发的表面增强拉曼基因（SERGen）探针可通过与互补DNA探针杂交来检测DNA靶标。这些探针无需使用放射性标记，具有提供灵敏度和选择性的巨大潜力。研究了该方法的有效性及其在癌症基因诊断（BRCA1乳腺癌基因）中的应用。