Vorob'ev E V, Perevozchikov A P
Ontogenez. 1992 Sep-Oct;23(5):469-79.
The apolipoprotein A-1 (apo A-1) gene expression at certain stages of human embryo development was studied using an in situ hybridization procedure. By the fifth week of development, the presence of apo A-1 mRNAs was detected in cells of different tissue types such as CNS rudiment, somite myotomes, ear vesicle, nasal placodes, lens, apical areas of the maxillary, mandibular and hyoid arcs, mesenchyme of limb buds, small intestine, mesonephros, genital ridge, and several types of blood cells. At later stages (weeks 8-9) the distribution of these mRNAs showed considerable changes. The high apo A-1 mRNA level was characteristic of liver cells, adrenals, kidney, neural tube and ganglia. These data suggest that changes in tissue-specific apo A-1 gene expression during human embryogenesis may be associated with the development of blood circulatory system and CNS.
利用原位杂交技术研究了人胚胎发育某些阶段载脂蛋白A-1（apo A-1）基因的表达。在发育的第五周，在不同组织类型的细胞中检测到apo A-1 mRNA的存在，如中枢神经系统原基、体节肌节、耳泡、鼻基板、晶状体、上颌、下颌和舌骨弓的顶端区域、肢芽间充质、小肠、中肾、生殖嵴以及几种类型的血细胞。在后期阶段（第8 - 9周），这些mRNA的分布显示出相当大的变化。高apo A-1 mRNA水平是肝细胞、肾上腺、肾脏、神经管和神经节的特征。这些数据表明，人类胚胎发生过程中组织特异性apo A-1基因表达的变化可能与血液循环系统和中枢神经系统的发育有关。
