西班牙鲍曼不动杆菌临床分离株中整合子介导的抗生素多重耐药性
Integron-mediated antibiotic multiresistance in Acinetobacter baumannii clinical isolates from Spain.
作者信息
Ruiz J, Navia M M, Casals C, Sierra J M, Jiménez De Anta M T, Vila J
机构信息
Institut Clinic d'Infeccions i Immunologia Clínica, IDIBAPS, Hospital Clínic, Facultat de Medicina, Universitat de Barcelona, Villaroel 170, 08036-Barcelona, Spain.
出版信息
Clin Microbiol Infect. 2003 Sep;9(9):907-11. doi: 10.1046/j.1469-0691.2003.00561.x.
OBJECTIVE
To determine whether non-epidemiologically related, antibiotic-resistant isolates of Acinetobacter baumannii from different geographical origins possess common type 1 integrons.
METHODS
The epidemiologic relationships between seven A. baumannii strains recovered from different Spanish hospitals were established by pulsed-field gel electrophoresis, the presence of integrons being determined by PCR and DNA sequencing.
RESULTS
Integron analysis showed the presence of four different integrons, containing six different known genes (aacC1, aacA4, aadA1, aadB, oxa21 and oxa37) plus an ORF. It was found that the same integron was present in different unrelated strains and that related strains could have different integrons.
CONCLUSION
These results show the potential risk of integron dissemination among different strains of A. baumannii.
目的
确定来自不同地理区域、非流行病学相关的鲍曼不动杆菌抗生素耐药菌株是否具有共同的1类整合子。
方法
通过脉冲场凝胶电泳确定从不同西班牙医院分离出的7株鲍曼不动杆菌之间的流行病学关系，通过聚合酶链反应（PCR）和DNA测序确定整合子的存在。
结果
整合子分析显示存在4种不同的整合子，包含6种不同的已知基因（aacC1、aacA4、aadA1、aadB、oxa21和oxa37）以及一个开放阅读框（ORF）。发现不同的不相关菌株中存在相同的整合子，且相关菌株可能具有不同的整合子。
结论
这些结果表明整合子在不同鲍曼不动杆菌菌株间传播存在潜在风险。
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