Suvas S, Vohra H, Agrewala J N
Immunology Laboratory, Institute of Microbial Technology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Clin Exp Immunol. 2003 Nov;134(2):232-7. doi: 10.1046/j.1365-2249.2003.02283.x.
M150 is an 150-kDa protein associated with the surface of macrophages and is responsible chiefly for the activation of Th1 cells. It is a unique subset of the lysosome-associated membrane protein-1 glycoprotein and its co-stimulatory activity depends on its post-translational modification, which has a distinct glycosylation pattern restricted to macrophages. In the present study, we have observed that M150 is expressed constitutively on peritoneal but not splenic macrophages isolated from mice of different genetic backgrounds: Balb/c, C57BL/6 and C3He. However, M150 was expressed not only on peritoneal but also on splenic macrophages of non-obese diabetic (NOD) mice. Expression on splenic macrophages was induced by culture with lipopolysaccharide (LPS). Expression could also be significantly up-regulated by interferon (IFN)-gamma and granulocyte-macrophage colony stimulating factor (GM-CSF) but was inhibited by interleukin (IL)-10; IL-4 exhibited no effect. Further, cross-linking of B7-2, CD40, ICAM-1 but not B7-1 enhanced the level of M150 significantly. IFN-gamma and GM-CSF acted synergistically with CD40. The significance of these findings is that cytokines IFN-gamma, GM-CSF and IL-10 and the co-stimulatory molecules B7-2, CD40 and ICAM-1 can regulate the expression of M150 on macrophages.
M150是一种与巨噬细胞表面相关的150千道尔顿蛋白质，主要负责Th1细胞的激活。它是溶酶体相关膜蛋白-1糖蛋白的一个独特亚群，其共刺激活性取决于其翻译后修饰，这种修饰具有仅限于巨噬细胞的独特糖基化模式。在本研究中，我们观察到M150在从不同遗传背景（Balb/c、C57BL/6和C3He）的小鼠中分离出的腹膜巨噬细胞上持续表达，但在脾巨噬细胞上不表达。然而，M150不仅在非肥胖糖尿病（NOD）小鼠的腹膜巨噬细胞上表达，也在其脾巨噬细胞上表达。脾巨噬细胞上的表达是通过用脂多糖（LPS）培养诱导的。干扰素（IFN）-γ和粒细胞-巨噬细胞集落刺激因子（GM-CSF）也可使其表达显著上调，但白细胞介素（IL）-10可抑制其表达；IL-4无作用。此外，B7-2、CD40、ICAM-1的交联可显著提高M150的水平，但B7-1交联则无此作用。IFN-γ和GM-CSF与CD40协同作用。这些发现的意义在于，细胞因子IFN-γ、GM-CSF和IL-10以及共刺激分子B7-2、CD40和ICAM-１可调节巨噬细胞上M150的表达。
