• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

体外有效进行载脂蛋白B（apoB）RNA编辑需要三种不同的RNA序列元件。

Three distinct RNA sequence elements are required for efficient apolipoprotein B (apoB) RNA editing in vitro.

作者信息

Backus J W, Smith H C

机构信息

Department of Biochemistry, University of Rochester, NY 14642.

出版信息

Nucleic Acids Res. 1992 Nov 25;20(22):6007-14. doi: 10.1093/nar/20.22.6007.

DOI:10.1093/nar/20.22.6007
PMID:1461733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334467/
Abstract

Apolipoprotein B (apoB) mRNA is edited in rat liver and intestine to convert a CAA glutamine codon to a UAA translational stop codon by the direct conversion of cytidine to uridine at nucleotide 6666. We have proposed the 'mooring sequence' model for apoB RNA editing, in which editing complexes (editosomes) assemble on specific apoB mRNA flanking sequences to direct this site-specific editing event. One sequence element (approx. nts 6671-81, the presumed 'mooring sequence') has been previously identified as necessary for editing. We have identified two additional sequence elements which are necessary for efficient editing: (1) a 5' 'Regulator' region which modulates editing efficiency and (2) a 'Spacer' region between the editing site and the 3' mooring sequence, whose distance is critical for efficient editing. Utilizing this data, we have induced editing at a cryptic site and have defined a 22 nucleotide 'cassette' of specific apoB sequence which is sufficient to support wild-type levels of editing in vitro in a background of distal apoB RNA sequence.

摘要

载脂蛋白B（apoB）信使核糖核酸（mRNA）在大鼠肝脏和肠道中发生编辑，通过在核苷酸6666处将胞苷直接转化为尿苷，把一个CAA谷氨酰胺密码子转变为UAA翻译终止密码子。我们已经提出了apoB RNA编辑的“停泊序列”模型，即编辑复合体（编辑体）在特定的apoB mRNA侧翼序列上组装，以指导这一特异位点的编辑事件。一个序列元件（约核苷酸6671 - 81，推测为“停泊序列”）先前已被确定为编辑所必需。我们还鉴定出另外两个对高效编辑必不可少的序列元件：（1）一个5'“调节”区域，可调节编辑效率；（2）编辑位点与3'停泊序列之间的一个“间隔”区域，其距离对高效编辑至关重要。利用这些数据，我们在一个隐蔽位点诱导了编辑，并确定了一个由22个核苷酸组成的特定apoB序列“盒”，在apoB RNA远端序列背景下，该序列足以在体外支持野生型水平的编辑。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/eda3587d31dc/nar00233-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/b98ebda1667b/nar00233-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/c3bf7dec9658/nar00233-0127-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/10391db48d1d/nar00233-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/54fa61797fa8/nar00233-0128-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/404857f20137/nar00233-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/07073cc09002/nar00233-0129-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/eda3587d31dc/nar00233-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/b98ebda1667b/nar00233-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/c3bf7dec9658/nar00233-0127-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/10391db48d1d/nar00233-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/54fa61797fa8/nar00233-0128-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/404857f20137/nar00233-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/07073cc09002/nar00233-0129-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2896/334467/eda3587d31dc/nar00233-0130-a.jpg

相似文献

1
Three distinct RNA sequence elements are required for efficient apolipoprotein B (apoB) RNA editing in vitro.体外有效进行载脂蛋白B（apoB）RNA编辑需要三种不同的RNA序列元件。
Nucleic Acids Res. 1992 Nov 25;20(22):6007-14. doi: 10.1093/nar/20.22.6007.
2
Apolipoprotein B mRNA sequences 3' of the editing site are necessary and sufficient for editing and editosome assembly.载脂蛋白B信使核糖核酸编辑位点3'端的序列对于编辑和编辑体组装而言是必要且充分的。
Nucleic Acids Res. 1991 Dec 25;19(24):6781-6. doi: 10.1093/nar/19.24.6781.
3
Specific 3' sequences flanking a minimal apolipoprotein B (apoB) mRNA editing 'cassette' are critical for efficient editing in vitro.
Biochim Biophys Acta. 1994 Jan 18;1217(1):65-73.
4
Phylogenetic analysis of the apolipoprotein B mRNA-editing region. Evidence for a secondary structure between the mooring sequence and the 3' efficiency element.载脂蛋白B信使核糖核酸编辑区域的系统发育分析。系泊序列与3' 效率元件之间二级结构的证据。
J Biol Chem. 1999 Dec 3;274(49):34590-7. doi: 10.1074/jbc.274.49.34590.
5
Only cytidines 5' of the apolipoprotein B mRNA mooring sequence are edited.
Biochim Biophys Acta. 1994 Sep 13;1219(1):1-14. doi: 10.1016/0167-4781(94)90240-2.
6
Apolipoprotein B RNA sequence 3' of the mooring sequence and cellular sources of auxiliary factors determine the location and extent of promiscuous editing.停泊序列3'端的载脂蛋白B RNA序列以及辅助因子的细胞来源决定了混杂编辑的位置和程度。
Nucleic Acids Res. 1998 Apr 1;26(7):1644-52. doi: 10.1093/nar/26.7.1644.
7
Sequence elements required for apolipoprotein B mRNA editing enhancement activity from chicken enterocytes.鸡肠上皮细胞中载脂蛋白B mRNA编辑增强活性所需的序列元件。
Biochem Biophys Res Commun. 1999 Jan 27;254(3):744-50. doi: 10.1006/bbrc.1998.9963.
8
Two efficiency elements flanking the editing site of cytidine 6666 in the apolipoprotein B mRNA support mooring-dependent editing.载脂蛋白B信使核糖核酸中胞嘧啶6666编辑位点两侧的两个效率元件支持系泊依赖性编辑。
J Biol Chem. 1998 Apr 17;273(16):9435-42. doi: 10.1074/jbc.273.16.9435.
9
Transgenic mice expressing full-length human apolipoprotein B-100. Full-length human apolipoprotein B mRNA is essentially not edited in mouse intestine or liver.表达全长人载脂蛋白B - 100的转基因小鼠。全长人载脂蛋白B信使核糖核酸在小鼠肠道或肝脏中基本不发生编辑。
J Biol Chem. 1992 Oct 25;267(30):21412-20.
10
Apolipoprotein B mRNA editing in 12 different mammalian species: hepatic expression is reflected in low concentrations of apoB-containing plasma lipoproteins.12种不同哺乳动物物种中的载脂蛋白B信使核糖核酸编辑：肝脏表达反映在含载脂蛋白B的血浆脂蛋白低浓度中。
J Lipid Res. 1993 Aug;34(8):1367-83.

引用本文的文献

1
Revealing the hidden RBP-RNA interactions with RNA modification enzyme-based strategies.揭示基于 RNA 修饰酶的策略的隐藏的 RBP-RNA 相互作用。
Wiley Interdiscip Rev RNA. 2024 May-Jun;15(3):e1863. doi: 10.1002/wrna.1863.
2
C-to-U RNA Editing: A Site Directed RNA Editing Tool for Restoration of Genetic Code.C 到 U RNA 编辑：一种用于恢复遗传密码的定点 RNA 编辑工具。
Genes (Basel). 2022 Sep 12;13(9):1636. doi: 10.3390/genes13091636.
3
APOBEC1 mediated C-to-U RNA editing: target sequence and trans-acting factor contribution to 177 RNA editing events in 119 murine transcripts in-vivo.

本文引用的文献

1
Apolipoprotein B-48 is the product of a messenger RNA with an organ-specific in-frame stop codon.载脂蛋白B-48是一种信使核糖核酸的产物，该信使核糖核酸带有一个器官特异性的框内终止密码子。
Science. 1987 Oct 16;238(4825):363-6. doi: 10.1126/science.3659919.
2
A novel form of tissue-specific RNA processing produces apolipoprotein-B48 in intestine.一种新型的组织特异性RNA加工方式在肠道中产生载脂蛋白B48。
Cell. 1987 Sep 11;50(6):831-40. doi: 10.1016/0092-8674(87)90510-1.
3
Construction of mutant and chimeric genes using the polymerase chain reaction.利用聚合酶链反应构建突变基因和嵌合基因。
载脂蛋白B mRNA编辑酶催化多肽样蛋白1介导的C到U RNA编辑：体内119个小鼠转录本中177个RNA编辑事件的靶序列和顺式作用因子贡献
RNA. 2021 Jun 3;27(8):876-90. doi: 10.1261/rna.078678.121.
4
RNA Editors, Cofactors, and mRNA Targets: An Overview of the C-to-U RNA Editing Machinery and Its Implication in Human Disease.RNA编辑酶、辅助因子与mRNA靶点：C到U RNA编辑机制概述及其在人类疾病中的意义
Genes (Basel). 2018 Dec 27;10(1):13. doi: 10.3390/genes10010013.
5
A Novel RNA Editing Sensor Tool and a Specific Agonist Determine Neuronal Protein Expression of RNA-Edited Glycine Receptors and Identify a Genomic APOBEC1 Dimorphism as a New Genetic Risk Factor of Epilepsy.一种新型RNA编辑传感器工具和一种特异性激动剂决定了RNA编辑的甘氨酸受体的神经元蛋白表达，并确定一种基因组载脂蛋白B mRNA编辑酶1（APOBEC1）二态性为癫痫的新遗传危险因素。
Front Mol Neurosci. 2018 Jan 11;10:439. doi: 10.3389/fnmol.2017.00439. eCollection 2017.
6
Hippocampal Characteristics and Invariant Sequence Elements Distribution of GLRA2 and GLRA3 C-to-U Editing.海马体特征以及GLRA2和GLRA3 C到U编辑的不变序列元件分布
Mol Syndromol. 2017 Mar;8(2):85-92. doi: 10.1159/000453300. Epub 2016 Dec 16.
7
RNA Editing-Systemic Relevance and Clue to Disease Mechanisms?RNA编辑——系统相关性与疾病机制线索？
Front Mol Neurosci. 2016 Nov 23;9:124. doi: 10.3389/fnmol.2016.00124. eCollection 2016.
8
RNA binding to APOBEC deaminases; Not simply a substrate for C to U editing.APOBEC 脱氨酶的 RNA 结合；不仅仅是 C 到 U 编辑的底物。
RNA Biol. 2017 Sep 2;14(9):1153-1165. doi: 10.1080/15476286.2016.1259783. Epub 2016 Nov 21.
9
Identification of DNA cleavage- and recombination-specific hnRNP cofactors for activation-induced cytidine deaminase.鉴定激活诱导胞苷脱氨酶的DNA切割和重组特异性hnRNP辅因子。
Proc Natl Acad Sci U S A. 2015 May 5;112(18):5791-6. doi: 10.1073/pnas.1506167112. Epub 2015 Apr 20.
10
Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells.C>U mRNA编辑的流式细胞术可视化揭示了活细胞中该过程的动态变化。
RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033.
Nucleic Acids Res. 1989 Jan 25;17(2):723-33. doi: 10.1093/nar/17.2.723.
4
Sequence requirements for apolipoprotein B RNA editing in transfected rat hepatoma cells.转染大鼠肝癌细胞中载脂蛋白B RNA编辑的序列要求
J Biol Chem. 1989 Aug 15;264(23):13395-8.
5
An in vitro system for the editing of apolipoprotein B mRNA.一种用于编辑载脂蛋白B信使核糖核酸的体外系统。
Cell. 1989 Aug 11;58(3):519-25. doi: 10.1016/0092-8674(89)90432-7.
6
RNA editing in plant mitochondria.植物线粒体中的RNA编辑
Nature. 1989 Oct 19;341(6243):662-6. doi: 10.1038/341662a0.
7
Extensive editing of the cytochrome c oxidase III transcript in Trypanosoma brucei.布氏锥虫细胞色素c氧化酶III转录本的广泛编辑
Cell. 1988 May 6;53(3):413-22. doi: 10.1016/0092-8674(88)90161-4.
8
RNA editing of apolipoprotein B mRNA. Sequence specificity determined by in vitro coupled transcription editing.载脂蛋白B信使核糖核酸的核糖核酸编辑。体外偶联转录编辑确定的序列特异性。
J Biol Chem. 1990 Apr 25;265(12):6811-6.
9
Apolipoprotein B mRNA editing. Direct determination of the edited base and occurrence in non-apolipoprotein B-producing cell lines.载脂蛋白B信使核糖核酸编辑。编辑碱基的直接测定及其在不产生载脂蛋白B的细胞系中的出现情况。
J Biol Chem. 1990 Dec 25;265(36):22446-52.
10
A 40 kilodalton rat liver nuclear protein binds specifically to apolipoprotein B mRNA around the RNA editing site.一种40千道尔顿的大鼠肝脏核蛋白在RNA编辑位点周围与载脂蛋白B信使核糖核酸特异性结合。
Nucleic Acids Res. 1990 Oct 11;18(19):5817-21. doi: 10.1093/nar/18.19.5817.