Fry R C
Victorian Institute of Animal Science, Werribee, Australia.
Reprod Fertil Dev. 1992;4(4):449-58. doi: 10.1071/rd9920449.
Leukaemia inhibitory factor (LIF) was originally identified as a haemopoetic factor that induced the differentiation of certain myeloid leukaemia cell lines. In contrast to this action, LIF was subsequently shown to inhibit the spontaneous differentiation of murine embryonic stem cells in culture, thus maintaining their pluripotency and ability to contribute to the germline of chimaeric mice. In the mouse, mRNA for LIF is expressed by the endometrial glands of the uterus coincident with the time of blastocyst implantation and receptors have been found on the preimplantation blastocyst. The signal for LIF expression appears to be of maternal origin, perhaps regulated by oestradiol. Recombinant LIF improves the development of murine and ovine blastocysts in culture although there is some species specificity with respect to the type of LIF that is bioactive. It is proposed here that LIF acts on the trophectoderm of the rapidly expanding blastocyst and improves the implantation rate of otherwise compromised embryos. Further studies in livestock should elicit therapeutic uses for LIF in embryo culture, embryo transfer and embryo survival in vivo.
白血病抑制因子（LIF）最初被鉴定为一种造血因子，可诱导某些髓系白血病细胞系的分化。与这种作用相反，LIF随后被证明可抑制培养中的小鼠胚胎干细胞的自发分化，从而维持其多能性以及对嵌合体小鼠种系的贡献能力。在小鼠中，LIF的mRNA在囊胚着床时由子宫内膜腺表达，并且在着床前的囊胚上发现了受体。LIF表达的信号似乎源自母体，可能受雌二醇调节。重组LIF可改善培养中的小鼠和绵羊囊胚的发育，尽管就具有生物活性的LIF类型而言存在一些物种特异性。本文提出，LIF作用于快速扩张的囊胚的滋养外胚层，并提高原本发育不良的胚胎的着床率。在牲畜上的进一步研究应能得出LIF在胚胎培养、胚胎移植和体内胚胎存活方面的治疗用途。
