鼠伤寒沙门氏菌LT2的分批培养：化学计量学及导致生长停止的因素
Batch growth of Salmonella typhimurium LT2: stoichiometry and factors leading to cessation of growth.
作者信息
Wilson P D G, Wilson D R, Brocklehurst T F, Coleman H P, Mitchell G, Waspe C R, Jukes S A, Robins M M
机构信息
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
出版信息
Int J Food Microbiol. 2003 Dec 31;89(2-3):195-203. doi: 10.1016/s0168-1605(03)00142-9.
Salmonella typhimurium LT2 was grown in batch culture (trypticase soy broth, with 0.3%(w/v) yeast extract, 1% (w/v) glucose and 0.5% (w/v/) NaCl, 20 degrees C) at a range of initial pH (4.4, 4.8, 5.0 and 7.0). The consumption of oxygen and glucose was found to be independent of initial pH, and stoichiometric with growth. Mean yield coefficients of 6.9 x 10(-15) and 15.5 x 10(-15) mol oxygen/cell were estimated. Calculation of the instantaneous state of carbon during the cultivation showed stoichiometric conversion of glucose into biomass, carbon dioxide and organic acids. The concentration of the undissociated form of the primary acidic product (acetic acid) was shown to be the factor limiting growth.
鼠伤寒沙门氏菌LT2在分批培养中（胰蛋白胨大豆肉汤，含0.3%（w/v）酵母提取物、1%（w/v）葡萄糖和0.5%（w/v）氯化钠，20℃），在一系列初始pH值（4.4、4.8、5.0和7.0）下培养。发现氧气和葡萄糖的消耗与初始pH值无关，且与生长呈化学计量关系。估计平均产率系数为6.9×10⁻¹⁵和15.5×10⁻¹⁵摩尔氧气/细胞。培养过程中碳的瞬时状态计算表明，葡萄糖化学计量转化为生物质、二氧化碳和有机酸。主要酸性产物（乙酸）未解离形式的浓度被证明是限制生长的因素。
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