Harder Thomas, Franke Kerstin, Fahrenkrog Sonja, Aerts Leona, Van Bree Rieta, Van Assche F André, Plagemann Andreas
Clinic of Obstetrics, Division of Experimental Obstetrics, Humboldt University Medical School (Charité), Campus Virchow-Klinikum, Augustenburger Platz 1, 13353, Berlin, Germany.
Neurosci Lett. 2003 Dec 11;352(3):163-6. doi: 10.1016/j.neulet.2003.08.066.
Exposure to gestational diabetes (GD) in rats leads to dysplasia of the ventromedial hypothalamic nucleus (VMN), decisively involved into the regulation of body weight and metabolism. Recently, we have shown here that VMN malformation is absent in adult offspring of GD mothers treated by pancreatic islet transplantation during gestation. We therefore now investigated whether VMN malformation and its prevention are already present at the early postnatal end of the critical hypothalamic differentiation period. Already at weaning, the total number of VMN neurons, the volume of the VMN relative to total brain volume, and the numerical density of neurons in the anterior subnucleus of the VMN were reduced in offspring of sham-transplanted mothers (all P<0.05), but did not differ between offspring of islet-transplanted mothers and controls. No morphometric alterations occurred in the paraventricular hypothalamic nucleus. In conclusion, prevention of VMN malformation in offspring of islet-transplanted diabetic mothers is a direct consequence of normalized maternal metabolism during critical perinatal development.
大鼠孕期暴露于妊娠糖尿病（GD）会导致腹内侧下丘脑核（VMN）发育异常，而VMN对体重和新陈代谢的调节起关键作用。最近，我们在此表明，孕期接受胰岛移植治疗的GD母亲的成年后代不存在VMN畸形。因此，我们现在研究了在关键的下丘脑分化期产后早期结束时，VMN畸形及其预防是否已经存在。在断奶时，假移植母亲的后代中VMN神经元总数、VMN相对于全脑体积的体积以及VMN前亚核中神经元的数值密度均降低（所有P<0.05），但胰岛移植母亲的后代与对照组之间没有差异。下丘脑室旁核未出现形态计量学改变。总之，胰岛移植糖尿病母亲的后代中VMN畸形的预防是围产期关键发育过程中母体代谢正常化的直接结果。
