Chen Li-Bing, Liu Tao, Wu Jing-Xiang, Chen Xiao-Feng, Wang Li, Fan Chun-Lin, Gao Ping-Jin, Higashino Hideaki, Lee Wen-Hsiung, Yuan Wen-Jun, Chen Hong
Department of Thoracic Surgery, Shanghai 85 Hospital, Shanghai 200052, China.
Acta Pharmacol Sin. 2003 Nov;24(11):1077-82.
To determine the effects of hypertonic solution on myocardial ischemia and reperfusion injury in normal and stroke-prone hypertensive rat hearts in vitro.
Hearts were perfused in an isolated-perfused Langendorff apparatus and perfused with normal or hypertonic solution (360 mOsm/L, by addition of NaCl to the normal perfusate of 300 mOsm/L) before subjected to 30 min ischemia followed by 40 min isotonic reperfusion. Heart function, myocardial creatine kinase leakage, norepinephrine release, and ventricular calcium content were determined.
Normal rat hearts with hypertonic perfusion showed higher recovery rate of spontaneous beating than control hearts after ischemia. Hypertensive rat hearts perfused with hypertonic solution also had better recovery in diastolic function and less creatine kinase leakage than hypertensive controls. Concomitantly, myocardial release of norepinephrine was also reduced from hypertensive hearts perfused with hypertonic solution. There was no significant difference in myocardial calcium content between normal and hypertonic perfused hypertensive hearts.
Hypertonic perfusion may precondition the hearts and protect them from ischemia and reperfusion injury in both normal and hypertensive rats. The modulation of hypertonic perfusion on myocardial norepinephrine release and its role in cardioprotection needs further investigation.
在体外确定高渗溶液对正常大鼠和易中风高血压大鼠心脏的心肌缺血及再灌注损伤的影响。
在离体灌注的Langendorff装置中对心脏进行灌注，在经历30分钟缺血然后40分钟等渗再灌注之前，用正常溶液或高渗溶液（360毫渗量浓度/升，通过向300毫渗量浓度/升的正常灌注液中添加氯化钠）进行灌注。测定心脏功能、心肌肌酸激酶泄漏、去甲肾上腺素释放和心室钙含量。
高渗灌注的正常大鼠心脏在缺血后显示出自发搏动的恢复率高于对照心脏。用高渗溶液灌注的高血压大鼠心脏在舒张功能方面也有更好的恢复，并且肌酸激酶泄漏比高血压对照组更少。同时，用高渗溶液灌注的高血压心脏的心肌去甲肾上腺素释放也减少。正常灌注和高渗灌注的高血压心脏之间的心肌钙含量没有显著差异。
高渗灌注可能对心脏起到预处理作用，并保护正常和高血压大鼠的心脏免受缺血及再灌注损伤。高渗灌注对心肌去甲肾上腺素释放的调节及其在心脏保护中的作用需要进一步研究。
