Yamafuji K, Matsui Y
Nakamura Gakuen College, Fukuoka, Japan.
Agents Actions Suppl. 1992;38 ( Pt 2):58-64.
The enzyme which could produce kinin-like peptides from rat plasma at acidic condition and at coexistence of thiol compound was separated from acid extract of rat spleen. The isolation procedure of this enzyme was established. Substrate specificity was examined against fluologenic substrates and two types of collagen. The purified enzyme was proved to have kinin-forming activity and also collagenlytic activity.
在酸性条件下，于硫醇化合物共存时，能从大鼠血浆中产生激肽样肽的酶从大鼠脾脏的酸提取物中分离得到。该酶的分离方法得以确立。针对荧光底物和两种类型的胶原蛋白检测了底物特异性。纯化后的酶被证明具有激肽生成活性以及胶原蛋白水解活性。
