Nakamura-Minami M, Furuichi Y, Ishikawa K, Mitsuzono-Tofuku Y, Izumi Y
Department of Periodontology, Kagoshima University Dental School, Sakuragaoka, Kagoshima, Japan.
Oral Dis. 2003 Sep;9(5):249-54. doi: 10.1034/j.1601-0825.2003.02884.x.
The possible contribution of alpha1-protease inhibitor (alpha1-PI) and secretory leukocyte protease inhibitor (SLPI) in gingival crevicular fluid (GCF) to predict the periodontal disease activity was evaluated.
GCF samples were collected at each site before scaling and root planning (SRP), 2 and 4 weeks after SRP.
Forty-one sites that initially bled on probing in 21 patients with moderate to severe periodontitis were studied. Sites were classified according to the presence or absence of bleeding on probing (BOP) at 4 weeks. In GCF alpha1-PI and SLPI were determined by enzyme-linked immunosorbent assays.
A significant decrease was observed in alpha1-PI at 2 and 4 weeks in BOP(-) sites and at 4 weeks in BOP(+) sites. SLPI significantly increased at 2 weeks in BOP(+) site, while SLPI did not significantly differ at both time points in BOP(-) sites and at 4 weeks in BOP(+) sites. GCF alpha1-PI was significantly less at 2 weeks in BOP(-) than in BOP(+) sites.
At 2 weeks GCF alpha1-PI may reflect the healing response of the periodontal tissues following nonsurgical periodontal treatment. GCF SLPI levels may be influenced by healing.
评估龈沟液（GCF）中α1-蛋白酶抑制剂（α1-PI）和分泌型白细胞蛋白酶抑制剂（SLPI）对预测牙周疾病活动的可能作用。
在龈上洁治和根面平整（SRP）前、SRP后2周和4周，在每个部位采集GCF样本。
对21例中重度牙周炎患者中最初探诊出血的41个部位进行研究。根据4周时探诊出血（BOP）的有无对部位进行分类。采用酶联免疫吸附测定法测定GCF中的α1-PI和SLPI。
在BOP（-）部位，2周和4周时α1-PI显著降低；在BOP（+）部位，4周时α1-PI显著降低。在BOP（+）部位，2周时SLPI显著升高，而在BOP（-）部位的两个时间点以及BOP（+）部位的4周时，SLPI无显著差异。在2周时，BOP（-）部位的GCF α1-PI显著低于BOP（+）部位。
在2周时，GCF α1-PI可能反映非手术牙周治疗后牙周组织的愈合反应。GCF SLPI水平可能受愈合的影响。
