Simple high-performance liquid chromatographic method for the determination of acyclovir in pharmaceuticals.

An assay method for the determination of acyclovir from pharmaceutical preparations has been developed for assessment of product quality utilising high-performance liquid chromatography. The chromatographic conditions comprised a reversed-phase C18 column (250 x 4.6 mm i.d.) with a mobile phase of acetonitrile-20 mmol l(-1) aqueous ammonium acetate buffer of pH 4.5 (40:60). The flow rate was 0.8 ml min(-1) and UV detection was used at 250 nm. Calibration graph was linear in the range 1.98-59.4 microg ml(-1). The method has been validated according to current guidelines including assay of pharmacopoeial standard tablets. Recoveries ranged from 96.64 to 99.53%. The exipients present in the tablets did not interfere with the method.