动力学分光光度法测定药物制剂和人血清中的某些含硫化合物。
Kinetic spectrophotometric determination of some sulfur containing compounds in pharmaceutical preparations and human serum.
作者信息
Walash M I, Metwally M E-S, El-Brashy A M, Abdelal A A
机构信息
Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt.
出版信息
Farmaco. 2003 Dec;58(12):1325-32. doi: 10.1016/S0014-827X(03)00167-8.
A kinetic spectrophotometric method was developed for the determination of carbocisteine, ethionamide, thioctic acid and penicillamine based on the catalytic effect on the reaction between sodium azide and iodine in aqueous solution. Ten to 100 microg ml(-1) of carbocisteine and ethionamide, 0.1-1 microg ml(-1) of thioctic acid and 0.01-0.1 microg ml(-1) of penicillamine could be determined, respectively, by measuring the decrease in the absorbance of iodine at 348 nm by a fixed time method. The decrease in the absorbance in the first 5 min from the initiation of the reaction is related to the concentration of the drugs. The detection limits were 0.47, 0.71, 0.018 and 9.38 x 10(-4) microg ml(-1) for the four drugs, respectively. The proposed procedure was successfully applied in the determination of these drugs in pharmaceutical preparations and human serum.
基于对水溶液中叠氮化钠与碘之间反应的催化作用，建立了一种动力学分光光度法用于测定羧甲司坦、乙硫异烟胺、硫辛酸和青霉胺。通过固定时间法测量348nm处碘吸光度的降低，分别可测定10至100μg ml⁻¹的羧甲司坦和乙硫异烟胺、0.1至1μg ml⁻¹的硫辛酸以及0.01至0.1μg ml⁻¹的青霉胺。反应开始后前5分钟吸光度的降低与药物浓度相关。四种药物的检测限分别为0.47、0.71、0.018和9.38×10⁻⁴μg ml⁻¹。所提出的方法成功应用于药物制剂和人血清中这些药物的测定。
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