一种用于测定药物制剂中磺胺类药物的灵敏分光光度法。

A sensitive spectrophotometric method for the determination of sulfonamides in pharmaceutical preparations.

作者信息

Nagaraja Padmarajaiah, Naik Shailendra D, Shrestha Ashwinee Kumar, Shivakumar Anantharaman

机构信息

Department of Studies in Chemistry, University of Mysore, Manasagangothri Mysore-570006, India.

出版信息

Acta Pharm. 2007 Sep;57(3):333-42. doi: 10.2478/v10007-007-0026-4.

DOI:10.2478/v10007-007-0026-4

PMID:17878112

Abstract

A new, simple and sensitive spectrophotometric method for the determination of some sulfonamide drugs has been developed. The method is based on the diazotization of sulfacetamide, sulfadiazine, sulfaguanidine, sulfamerazine, sulfamethazine, sulfamethoxazole, and their coupling with 8-hydroxyquinoline in alkaline media to yield red coloured products with absorption maxima at 500 nm. Beer's law is obeyed from 0.1-7.0 microg mL-1. The limits of quantification and limits of detection were 0.11-0.18 and 0.03-0.05 microg mL-1, respectively. Intraday precision (RSD 0.1-0.5%) and accuracy (recovery 97.3--100.8%) of the developed method were evaluated. No interference was observed from common adjuvants. The method has been successfully applied to the assay of sulpha drug in pharmaceutical formulations.

摘要

已开发出一种用于测定某些磺胺类药物的新型、简单且灵敏的分光光度法。该方法基于磺胺醋酰、磺胺嘧啶、磺胺胍、磺胺甲基嘧啶、磺胺二甲嘧啶、磺胺甲恶唑的重氮化反应，以及它们在碱性介质中与8-羟基喹啉偶联，生成在500nm处有最大吸收的红色产物。在0.1 - 7.0μg mL-1范围内符合比尔定律。定量限和检测限分别为0.11 - 0.18和0.03 - 0.05μg mL-1。对所开发方法的日内精密度（相对标准偏差0.1 - 0.5%）和准确度（回收率97.3 - 100.8%）进行了评估。未观察到常见辅料的干扰。该方法已成功应用于药物制剂中磺胺类药物的测定。