雌激素受体α和17β-羟基类固醇脱氢酶4在睫状体中的表达。
Expression of estrogen receptor alpha and 17beta-hydroxysteroid dehydrogenase 4 in the ciliary body.
作者信息
Kobayashi Kaori, Iwakiri Ryo, Kobayashi Hiroshi, Okinami Satoshi
机构信息
Department of Ophthalmology, Saga Medical School, 5-1-1 Nabeshima, 849-8501, Saga , Japan.
出版信息
Graefes Arch Clin Exp Ophthalmol. 2004 Feb;242(2):172-176. doi: 10.1007/s00417-003-0799-1. Epub 2003 Nov 21.
PURPOSE
To investigate the expression and distribution of estrogen receptor alpha (ERalpha) and 17beta-hydroxysteroid dehydrogenase 4 (HSD4) in bovine and monkey ciliary body.
METHODS
Immunohistochemical study was employed to investigate the expression of ERalpha and 17beta-HSD4. To detect ERalpha and 17beta-HSD4 transcripts, reverse transcription-polymerase chain reaction (RT-PCR) was used.
RESULTS
In bovine and monkey ciliary body, the staining of the anti-ERalpha antibody was distributed in nonpigmented epithelium, and was not detectable in pigmented epithelium. Vessels in the stroma were also stained. The expression of 17beta-HSD4 was observed in nonpigmented and pigmented epithelium. RT-PCR revealed that the transcripts of ERalpha and 17beta-HSD4 were expressed in monkey ciliary body.
CONCLUSIONS
This study provides evidence for the presence of ERalpha and 17beta- HSD4 in the ciliary body. The presence of ERalpha and 17beta-HSD4 suggests that estrogen may have important functions in the ciliary body.
目的
研究雌激素受体α（ERα）和17β-羟基类固醇脱氢酶4（HSD4）在牛和猴睫状体中的表达及分布。
方法
采用免疫组织化学研究来检测ERα和17β-HSD4的表达。运用逆转录-聚合酶链反应（RT-PCR）来检测ERα和17β-HSD4转录本。
结果
在牛和猴的睫状体中，抗ERα抗体染色分布于无色素上皮，色素上皮未检测到。基质中的血管也被染色。在无色素和色素上皮中均观察到17β-HSD4的表达。RT-PCR显示ERα和17β-HSD4转录本在猴睫状体中表达。
结论
本研究为睫状体中存在ERα和17β-HSD4提供了证据。ERα和17β-HSD4的存在表明雌激素可能在睫状体中具有重要功能。
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