Alisch A, Rüping K, Köster F, Schöpper B, Baum M, Finas D, Felberbaum R, Dor S, Al-Hasani S, Diedrich K
Bereich Reproduktionsmedizin und gynäkologische Endokrinologie, Klinik für Frauenheilkunde und Geburtshilfe, Universität zu Lübeck.
Zentralbl Gynakol. 2003 Nov;125(11):452-7. doi: 10.1055/s-2003-44574.
To be able to predict the success of ART reliable tests for determining the quality of the oocytes are necessary. Apart from a vague morphologic assessment via microscopy a direct analysis of the oocyte quality is not possible. Because of the very close relation between the oocyte and the cumulus cells the analysis of the cumulus cells might give sufficient information on the oocyte quality. In this study we correlate the apoptotic activity of cumulus cells to the outcome of fertilized oocytes after Intracytoplasmic Sperm Injection (ICSI).
246 cumulus-oocyte-complexes from patients undergoing infertility treatment with the ICSI procedure were individually collected. The comet assay was used to determine the proportion of apoptotic cells within the cumulus population of each oocyte and correlated with oocyte fertilization and oocyte quality as well as with pregnancy outcome in 86 patients.
We were able to show that high quality embryos correlate to a low rate of apoptotic cells in their corresponding cumuli. Differences regarding the pregnancy outcome were statistically not significant.
Our results on cumulus cell apoptosis and embryo quality confirm other publications. To arrive at statistically proven criteria for the further development of single oocytes an increase in the number of analyzed patients is necessary.
为了能够可靠地预测辅助生殖技术（ART）的成功率，用于确定卵母细胞质量的检测方法是必要的。除了通过显微镜进行模糊的形态学评估外，无法对卵母细胞质量进行直接分析。由于卵母细胞与卵丘细胞之间关系密切，对卵丘细胞的分析可能会提供有关卵母细胞质量的足够信息。在本研究中，我们将卵丘细胞的凋亡活性与卵胞浆内单精子注射（ICSI）后受精卵母细胞的结果相关联。
分别收集了246个接受ICSI治疗的不育患者的卵丘-卵母细胞复合体。采用彗星试验确定每个卵母细胞卵丘群体中凋亡细胞的比例，并与86例患者的卵母细胞受精、卵母细胞质量以及妊娠结局相关联。
我们能够证明高质量胚胎与其相应卵丘中低凋亡细胞率相关。妊娠结局方面的差异在统计学上不显著。
我们关于卵丘细胞凋亡和胚胎质量的结果证实了其他文献。为了得出经统计学验证的单卵母细胞进一步发育标准，有必要增加分析患者的数量。
