Inui Madoka, Adachi Tetsuya, Takenaka Shigeo, Inui Hiroshi, Nakazawa Masami, Ueda Mitsuhiro, Watanabe Hajime, Mori Chisato, Iguchi Taisen, Miyatake Kazutaka
Department of Applied Biological Chemistry, Osaka Prefecture University, Osaka 599-8531, Japan.
Toxicology. 2003 Dec 15;194(1-2):43-50. doi: 10.1016/s0300-483x(03)00340-8.
Ultra violet (UV) screens and preservatives are widely and increasingly used in cosmetics and pharmaceuticals. In the present study, we examined the estrogenicity of 4-methyl-benzylidene camphor (4-MBC), octyl-methoxycinnamate (OMC), and propyl paraben (n-propyl-p-hydroxy-benzoate; PP), among UV screens and preservatives, using male medaka (Oryzias latipes), in regard to production of vitellogenin (VTG) and choriogenin (CHG) which are known to be estrogen-responsive gene products. First, using a VTG enzyme-linked immunosorbent assay (ELISA) system, we determined the increase in VTG plasma concentration in medaka due to exposure to 4-MBC, OMC, and PP, and compared this concentration to the non-treated control. Next, we found increases in mRNA expression levels of VTG subtypes VTG-1 and VTG-2, and CHG subtypes CHG-L and CHG-H, in liver due to exposure to 4-MBC, OMC, and PP compared to the non-treated control. In addition, we also found increased mRNA expression levels of estrogen receptor (ER) alpha, among sex hormone receptors in the liver, due to exposure to 4-MBC, OMC, and PP compared to the non-treated control. In this study, we showed that 4-MBC, OMC, and PP have estrogenic activity in fish.
紫外线（UV）防护剂和防腐剂在化妆品和药品中被广泛且越来越多地使用。在本研究中，我们使用雄性青鳉（Oryzias latipes），就已知为雌激素反应性基因产物的卵黄蛋白原（VTG）和卵壳蛋白原（CHG）的产生，检测了紫外线防护剂和防腐剂中的4-甲基亚苄基樟脑（4-MBC）、桂皮酸盐（OMC）和对羟基苯甲酸丙酯（正丙基-对羟基-苯甲酸盐；PP）的雌激素活性。首先，我们使用VTG酶联免疫吸附测定（ELISA）系统，测定了青鳉因接触4-MBC、OMC和PP而导致的血浆中VTG浓度的增加，并将该浓度与未处理的对照组进行比较。接下来，我们发现与未处理的对照组相比，由于接触4-MBC、OMC和PP，肝脏中VTG亚型VTG-1和VTG-2以及CHG亚型CHG-L和CHG-H的mRNA表达水平增加。此外，我们还发现与未处理的对照组相比，由于接触4-MBC、OMC和PP，肝脏中性激素受体中的雌激素受体（ER）α的mRNA表达水平增加。在本研究中，我们表明4-MBC、OMC和PP在鱼类中具有雌激素活性。
