Beytut E, Yuce A, Kamiloglu N N, Aksakal M
Department of Physiology, Veterinary Faculty of Kaskas University, Kars/Turkey.
Int J Vitam Nutr Res. 2003 Oct;73(5):351-5. doi: 10.1024/0300-9831.73.5.351.
The aim of this work was to determine the effect of dietary vitamin E intake on lipid peroxidation (LPO) by measuring thiobarbituric acid reactive substances (TBARS), vitamin E and reduced glutathione (GSH) levels, and glutathione peroxidase (GSH-Px: EC 1.11.1.9) activity in plasma, red blood cells (RBC), livers, and kidneys of rabbits dosed with cadmium (Cd). Six-month-old clinically healthy New Zealand White rabbits (8 in each group) were given tap water only, containing 1 g CdCl2/L, or tap water with CdCl2 plus vitamin E (100 mg dl-alpha-tocopheryl acetate in 0.2 mL corn oil) daily for 30 days. The vitamin E level in the plasma, liver, and kidneys was significantly higher in the control than in the Cd-only group, and TBARS levels were significantly lower. There were no statistical differences between the control and Cd-only groups GSH-Px activities and GSH levels in RBC, liver, and kidneys. Vitamin E levels in plasma, liver, and kidneys and GSH-Px activity in RBC were higher in the vitamin E group than in both control and Cd-only groups. However, the TBARS levels of RBC, liver, and kidneys in vitamin E administered group were decreased. Therefore, the present study demonstrates the effectiveness of vitamin E in reducing oxidative stress in Cd-treated rabbits and suggests that reductions in increased TBARS due to Cd toxicity may be an important factor in the action of vitamin E.
本研究的目的是通过测量镉（Cd）染毒家兔血浆、红细胞（RBC）、肝脏和肾脏中的硫代巴比妥酸反应物质（TBARS）、维生素E、还原型谷胱甘肽（GSH）水平以及谷胱甘肽过氧化物酶（GSH-Px：EC 1.11.1.9）活性，来确定膳食维生素E摄入量对脂质过氧化（LPO）的影响。将6月龄临床健康的新西兰白兔（每组8只）分别给予仅含1 g CdCl2/L的自来水、含CdCl2的自来水加维生素E（0.2 mL玉米油中含100 mg dl-α-生育酚醋酸酯），每日给药，持续30天。对照组血浆、肝脏和肾脏中的维生素E水平显著高于仅染镉组，且TBARS水平显著更低。对照组与仅染镉组RBC、肝脏和肾脏中的GSH-Px活性和GSH水平无统计学差异。维生素E组血浆、肝脏和肾脏中的维生素E水平以及RBC中的GSH-Px活性均高于对照组和仅染镉组。然而，给予维生素E组RBC、肝脏和肾脏中的TBARS水平降低。因此，本研究证明了维生素E在减轻镉处理家兔氧化应激方面的有效性，并表明降低因镉毒性导致的TBARS升高可能是维生素E发挥作用的一个重要因素。
