Wang Qin, Crofts Antony R, Padua Graciela W
Department of Food Science and Human Nutrition, University of Illinois, 382/D AESB, 1304 West Pennsylvania Avenue, Urbana, Illinois 61801.
J Agric Food Chem. 2003 Dec 3;51(25):7439-44. doi: 10.1021/jf0340658.
Experiments on the adsorption of alpha-zein (characterized by SDS-PAGE) from aqueous ethanol and 2-propanol solutions onto hydrophilic and hydrophobic surfaces are reported. Zein adsorption onto self-assembled monolayers (SAMs) was detected by surface plasmon resonance (SPR). Gold substrates were prepared by thermal evaporation on glass slides. Gold-coated surfaces were modified by depositing SAMs of either a long-chain carboxylic acid terminated thiol [COOH(CH2)(10)SH] or a methyl-terminated alkanethiol [CH3(CH2)(7)SH]. Experimental measurements indicated that zein interacted with both hydrophilic and hydrophobic surfaces. Zein concentration affected the thickness of bound zein layers. The estimated thickness of the zein monolayer deposited on hydrophilic surfaces was 4.7 nm. Zein monolayer thickness on hydrophobic surfaces was estimated at 4.6 nm. The topography of zein layers was examined by atomic force microscopy (AFM) after solvent was evaporated. Surface features of zein deposits depended on the adsorbing surface. On hydrophilic surfaces, roughness values were high and distinct ring-shaped structures were observed. On hydrophobic surfaces, zein formed a uniform and featureless coverage.
本文报道了关于α-玉米醇溶蛋白（通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳表征）从乙醇水溶液和2-丙醇溶液吸附到亲水和疏水表面的实验。通过表面等离子体共振（SPR）检测玉米醇溶蛋白在自组装单分子层（SAMs）上的吸附情况。通过热蒸发在载玻片上制备金基底。通过沉积长链羧酸封端的硫醇[COOH(CH2)(10)SH]或甲基封端的烷硫醇[CH3(CH2)(7)SH]的自组装单分子层对镀金表面进行修饰。实验测量表明，玉米醇溶蛋白与亲水和疏水表面均发生相互作用。玉米醇溶蛋白浓度影响结合的玉米醇溶蛋白层的厚度。沉积在亲水表面的玉米醇溶蛋白单层的估计厚度为4.7纳米。疏水表面上玉米醇溶蛋白单层厚度估计为4.6纳米。溶剂蒸发后，通过原子力显微镜（AFM）检查玉米醇溶蛋白层的形貌。玉米醇溶蛋白沉积物的表面特征取决于吸附表面。在亲水表面上，粗糙度值较高，观察到明显的环形结构。在疏水表面上，玉米醇溶蛋白形成均匀且无特征的覆盖层。
