Sinitsyna O A, Bukhtoyarov F E, Gusakov A V, Okunev O N, Bekkarevitch A O, Vinetsky Yu P, Sinitsyn A P
Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia.
Biochemistry (Mosc). 2003 Nov;68(11):1200-9. doi: 10.1023/b:biry.0000009134.48246.7e.
The composition of the enzyme complex secreted by Penicillium canescens was investigated. A scheme for purification of the main components of the complex by chromatofocusing on a Mono P column was developed. It was found that along with beta-galactosidase, the major components of the complex were endo-beta-1,4-xylanase (31 kD, pI 8.2-9.3), alpha-L-arabinofuranosidase (60 kD, pI 7.6), arabinoxylan-arabinofuranohydrolase (70 kD, pI 3.8-4.0), and endo-beta-1,3/1,4-glucanase (40 kD, pI 4.4). The substrate specificity, pH and temperature activity optima, adsorbability, thermal stability, and ability for synergic interaction of the isolated enzymes were studied.
