人牙髓中的淋巴管生成
Lymphangiogenesis in human dental pulp.
作者信息
Pimenta F J G S, Sá A R, Gomez R S
机构信息
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
出版信息
Int Endod J. 2003 Dec;36(12):853-6. doi: 10.1111/j.1365-2591.2003.00728.x.
AIM
To investigate the impact of inflammation on lymphangiogenesis in human dental pulp.
METHODOLOGY
Eleven samples of dental pulp without inflammation and 11 dental pulps with moderate to intense mononuclear cell inflammatory infiltrate associated with dentine caries were selected. The streptavidin-biotin complex stain was used to detect CD31, vascular endothelial growth factor receptor-3 (VEGFR-3) and alpha-smooth muscle actin. The number of lymphatic vessels was obtained by counting the number of vessels positive for CD31 and VEGFR-3 and negative for alpha-smooth muscle actin.
RESULTS
The results demonstrated that the mean number (+/-SD) of vessels positive for CD31 and VEGFR-3 (lymphatic vessels) in the group with inflammation (6.09 +/- 1.81) was statistically higher (P = 0.0123) than the mean number in the group without inflammation (3.73 +/- 2.20).
CONCLUSION
Increased co-immunostaining of CD31 and VEGF-3 in vessels associated with human dental pulp inflammation occurred, which suggests lymphangiogenesis.
目的
研究炎症对人牙髓淋巴管生成的影响。
方法
选取11个无炎症的牙髓样本和11个与牙本质龋相关的有中度至重度单核细胞炎性浸润的牙髓样本。采用链霉亲和素-生物素复合物染色法检测CD31、血管内皮生长因子受体-3（VEGFR-3）和α-平滑肌肌动蛋白。通过计数CD31和VEGFR-3阳性且α-平滑肌肌动蛋白阴性的血管数量来确定淋巴管数量。
结果
结果表明，有炎症组中CD31和VEGFR-3阳性的血管（淋巴管）平均数量（±标准差）为6.09±1.81，显著高于无炎症组的平均数量（3.73±2.20）（P = 0.0123）。
结论
在与人牙髓炎症相关的血管中，CD31和VEGF-3的共免疫染色增加，提示淋巴管生成。
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