重组大肠杆菌菌株中异源蛋白表达的傅里叶变换红外光谱研究
FT-IR study of heterologous protein expression in recombinant Escherichia coli strains.
作者信息
Ami Diletta, Bonecchi Loredana, Calì Simona, Orsini Gaetano, Tonon Giancarlo, Doglia Silvia Maria
机构信息
INFM UdR Milano-Bicocca, Department of Biotechnologies and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
出版信息
Biochim Biophys Acta. 2003 Dec 5;1624(1-3):6-10. doi: 10.1016/j.bbagen.2003.09.008.
Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm(-1) allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological interest for studying inclusion body formation.
通过傅里叶变换红外（FT-IR）显微光谱法对两种表达不同水平干扰素融合蛋白且以包涵体形式存在的重组大肠杆菌菌株进行了研究。1628 cm(-1)处的一条特征带能够通过对细胞沉淀进行直接分析，以快速、非侵入性且定量的方式监测蛋白质表达。结果表明，FT-IR显微光谱法是一种在研究包涵体形成方面具有潜在生物技术应用价值的技术。
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