Kharlamov E A, Jukkola P I, Schmitt K L, Kelly K M
Department of Neurology, Allegheny-Singer Research Institute, Allegheny General Hospital, 940 South Tower, 320 E. North Avenue, Pittsburgh, PA 15212-4772, USA.
Epilepsy Res. 2003 Oct;56(2-3):185-203. doi: 10.1016/j.eplepsyres.2003.09.005.
The goal of this study was to characterize the electroencephalographic (EEG) and behavioral properties of young adult rats during extended video-EEG monitoring following photothrombotic brain infarction. Two-month-old male Sprague-Dawley rats underwent photothrombotic brain infarction of the left sensorimotor cortex with the photosensitive dye rose bengal (n=10) or were used as controls (n=9). Qualitative and quantitative EEG analysis was performed on digital video-EEG records obtained during 6 months of recording. The main finding of this study was that 5/10 (50%) lesioned animals developed focal epileptic seizures ipsilateral to the cortical infarct characterized by rhythmic spike-wave discharges with or without behavioral change. Epileptic animals demonstrated increased delta, theta, and low beta-range power ipsilateral to the infarct that reliably distinguished them from lesioned nonepileptic and control animals. Lesioned animals (epileptic and nonepileptic) also demonstrated a distinct pattern of focal rhythmic theta activity before or after generalized high beta-range discharges. Electrical and behavioral characteristics common to both lesioned and control animals included: (1) focal rhythmic theta activity in either hemisphere; (2) focal low beta-range discharges in either hemisphere; (3) generalized high beta-range discharges; (4) absence seizures; (5) generalized pseudoperiodic spike discharges associated with mild multifocal body jerks; (6) tonic-clonic seizures (one nai;ve control; one lesioned animal). Cresyl violet staining of lesioned animals' brains showed consistent infarcts of the sensorimotor cortex extending to the subcortical white matter. These results provide an expanded electrobehavioral description of young adult rats following photothrombotic brain infarction and augment further investigation into the molecular, cellular, and network alterations that contribute to the establishment of post-stroke epilepsy.
本研究的目的是在光血栓性脑梗死之后的长时间视频脑电图监测期间，对年轻成年大鼠的脑电图（EEG）和行为特性进行表征。两个月大的雄性Sprague-Dawley大鼠，用光敏染料孟加拉玫瑰红对左侧感觉运动皮层进行光血栓性脑梗死（n = 10），或用作对照（n = 9）。对在6个月记录期间获得的数字视频脑电图记录进行定性和定量脑电图分析。本研究的主要发现是，10只损伤动物中有5只（50%）出现了皮层梗死同侧的局灶性癫痫发作，其特征为有或无行为改变的节律性棘波放电。癫痫动物梗死同侧的δ波、θ波和低β频段功率增加，这可靠地将它们与损伤的非癫痫动物和对照动物区分开来。损伤动物（癫痫和非癫痫）在全身性高β频段放电之前或之后也表现出一种独特的局灶性节律性θ活动模式。损伤动物和对照动物共有的电和行为特征包括：（1）任一脑半球的局灶性节律性θ活动；（2）任一脑半球的局灶性低β频段放电；（3）全身性高β频段放电；（4）失神发作；（5）与轻度多灶性身体抽搐相关的全身性假周期性棘波放电；（6）强直阵挛性发作（1只未处理对照；1只损伤动物）。损伤动物大脑的甲酚紫染色显示感觉运动皮层有一致的梗死灶，延伸至皮质下白质。这些结果提供了光血栓性脑梗死之后年轻成年大鼠的扩展电行为描述，并促进了对促成中风后癫痫形成的分子、细胞和网络改变的进一步研究。
